| 慢性乙型肝炎患者肝组织、淋巴细胞内HBV－DNA的检测及相关性研究 |
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| 引用本文： | 高丰光,孙汶生,曹英林,张利宁,宋静. 慢性乙型肝炎患者肝组织、淋巴细胞内HBV－DNA的检测及相关性研究[J]. 山东大学学报(医学版), 1996, 0(2) |
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| 作者姓名： | 高丰光 孙汶生 曹英林 张利宁 宋静 |
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| 作者单位： | Dept. of Microbiology，Shandong Medical University |
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| 摘 要： | 用分子生物学方法检测了乙型肝炎３７例的血清、外周血淋巴细胞及肝组织中的ＨＢＶ－ＤＮＡ存在情况，阳性率分别是５７．１％、８７．１％和８３．８％。统计分析表明，血清与外周血淋巴细胞、血清与肝组织中的ＨＢＶ－ＤＮＡ阳性率差异有显著性（Ｐ＜０．０１，Ｐ＜０．０５），外周血淋巴细胞与肝组织中的ＨＢＶ－ＤＮＡ阳性率的相关系数是０．８１（Ｐ＜０．０５），提示外周血淋巴细胞与肝组织中的ＨＢＶ－ＤＮＡ有一定的相关性。
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| 关 键 词： | 乙型肝炎；外周血淋巴细胞；乙型肝炎病毒；探针；核酸杂交 |
DETECTION HBV DNA IN LIVER TISSUES AND PERIPHERAL BLOOD LEUKOCYTES OF CHRONIC HEPATITIS B PATIENTS AND ITS CORRELATIONSHIP RESEARCH |
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| Gao Fengguang, Sun Wensheng, Cao Yingling,et al. DETECTION HBV DNA IN LIVER TISSUES AND PERIPHERAL BLOOD LEUKOCYTES OF CHRONIC HEPATITIS B PATIENTS AND ITS CORRELATIONSHIP RESEARCH[J]. Journal of Shandong University:Health Sciences, 1996, 0(2) |
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| Authors: | Gao Fengguang Sun Wensheng Cao Yingling et al |
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| Abstract: | HBV-DNA in serum, peripheral blood leukocytes(PBL)and liver tissues of 37 chronic hepatitis B patients was detected by molecular biological methods. The results showed: 1.The positive rate was 57.1%, 87.1% and 83.8%,respectively.2. There was obvious difference between serum group and PBL group,serum group and liver tissues group,(P<0. 01 and P<0.05, respectively). 3. The correlationship of HBV DNA in PBL and liver tissues also can be seen from the results (P<0. 05,r=0. 81).The results indicated that PBL HBV-DNA can be used to deduce the existence of HBV-DNA in liver. |
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| Keywords: | Hepatitis B Peripheral blood leukocytes Hepatitis B virus Probe Nucleic acid hybridization |
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