大肠癌患者外周血CK20表达及p53基因突变的研究

目的建立高效、快速的外周血中细胞角蛋白20(Cytokeratin 20，CZK20)表达的检测方法，探讨大肠癌患者外周血中CK20的表达与微转移的关系，研究大肠癌与p53基因突变的关系。方法运用荧光监测技术结合RT—PCR，检测36例大肠癌患者术前和术后不同时间外周血中的CK20的表达。用SSCP技术对大肠癌患者进行p53基因的突变检测。结果36例患者的外周血中检出CK20表达的有27例，阳性率为75％，手术后24h患者外周血中检出CK20表达的有30例，阳性率为83.3％，术后早期化疗结束后仅13例为阳性，阳性率为36％。36例患者的p53基因的SSCP突变检测中未检测到突变。结论运用荧光PCR技术检测外周血中CK20的表达可以监测癌症微转移的发生，简便快速，检出率高。

Study of Cytokeratin 20 Expression and p53 Gene Mutation in Peripheral Blood of Patients with Colorectal Carcinoma

Objective To set up an efficient and rapid method for detecting CK20 expression in peripheral blood of patients with colorectal carcinoma, and explore the relationship between CK20 expression and micrometastasis of colorectal carcinoma. Methods CK20 mRNA expression in peripheral blood of 36 patients with colorectal carcinomas was detected at various time points before and after operation by fluorescent RT-PCR. The mutation of p53 gene was also detected using SSCP. Results The positive rate of CK20 mRNA expression in peripheral blood before and 24 hours after operation was 75%(27/36) and 83.3%(30/36), respectively. After the short-term chemotherapy, the positive rate of CK20 mRNA expression was 36%(13/36). No mutation of p53 gene was found in those patients. Conclusion Fluorescent RT-PCR for detecting CK20 mRNA expression in peripheral blood was simple and efficacious. It was useful to monitor micrometastasis of colorectal carcinoma.