| 人骨形成蛋白纳米微粒的制备与生物活性鉴定 |
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| 引用本文： | 季晓风,丛宪玲,杨晓玉,段德生. 人骨形成蛋白纳米微粒的制备与生物活性鉴定[J]. 中国实验诊断学, 2006, 10(3): 261-263 |
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| 作者姓名： | 季晓风 丛宪玲 杨晓玉 段德生 |
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| 作者单位： | 吉林大学中日联谊医院,吉林,长春,130033 |
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| 摘 要： | 目的制备重组人骨形成蛋白-2（rhBMP-2）聚氰基丙烯酸正丁酯纳米微球缓释系统，检测其对兔骨髓间充质干细胞（BMSCs）的生物学效应。方法采用改良的乳化溶液聚合法制备rhBMP-2纳米微球；采用MTT法检测微球对BMSCs增殖的影响；碱性磷酸酶（ALP）检测试剂盒检测细胞ALP活性。结果该微球缓释系统具有一定的生物活性，可促进BMSCs的增殖，并呈剂量依赖性。其效应高于单纯施加rhBMP-2的效应。结论纳米材料是一种较好的缓释系统，作用效果优于单纯使用rhBMP-2。
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| 关 键 词： | 纳米微球 骨髓间充质干细胞 |
| 文章编号： | 1007-4287（2006）03-0261-03 |
| 收稿时间： | 2005-04-01 |
| 修稿时间： | 2005-04-01 |
Study on preparation and identification of nanometer particle of human bone morphogenetic protein |
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| JI Xiao-feng, CONG Xian-ling , YANG Xiao-yu ,et al.. Study on preparation and identification of nanometer particle of human bone morphogenetic protein[J]. Chinese Journal of Laboratory Diagnosis, 2006, 10(3): 261-263 |
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| Authors: | JI Xiao-feng CONG Xian-ling YANG Xiao-yu et al. |
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| Affiliation: | JI Xiao-feng, CONG Xian-ling , YANG Xiao-yu , et al. |
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| Abstract: | Objective To Prepare slow-release system of recombined human bone morphogentic protein-2(rhBMP-2) combined with and detect its the biological effects on rabbit bone marrow mesenchymal stem cells(BMSCs).Methods poly-emulsionized solution was used to prepare the nanometer particle of rhBMP-2,the effects of Nanometer Particle on the proliferation of BMSCs were detected by MTT and the activities of alkaline phosphatase(ALP) were detected by ALP test kit.Results The slow-release system established possessed the biological activity to enhance the proliferation of BMSCs and its effects is dose dependent.Conclusion The effects of slow-release system of rhBMP-2 combined with nanometer particle is better than that of rhBMP-2 alone. |
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| Keywords: | rhBMP-2 |
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