消炎痛对hCG诱发家兔排卵抑制的形态学研究

应用非甾体抗炎药消炎痛(20mg/kg)抑制hCG 诱发的家兔排卵,注射后的6、9、12、72小时剖腹观察排卵数,并取材进行透射电镜观察和酶组织化学观察。甾体激素合成酶类的组织化学和颗粒细胞内某些细胞器的电镜立体定量学测定结果表明:消炎痛抑制了排卵,却不影响颗粒细胞向黄体细胞的转变。透射电镜观察表明:在排卵前,实验组和对照组最明显的不同是:实验组的外膜和白膜很少见到胶原溶解现象,且表面上皮细胞内含的致密小体不释放其内容物。结果提示:消炎痛主要作用于排卵前卵泡顶部的成纤维细胞和表面上皮细胞,这可能与其抑制了某些与排卵有关的酶的释放有关。

A MORPHOLOGICAL STUDY ON THE INHIBITION OF HCGINDUCED OVULATION BY INDOMETHACIN IN RABBITS

hCG-induced rabbit ovulation was inhibited by ind(?)ethacin,a non-steroid antiinflam-matory drug,administered 6,9,12,72 hours after hCG injection.Ovaries were examinedfor counting the number of ovulation and removed for the study of enzyme histochemistry andtransmission electron microscopy.According to the histochemistry of some enzymes of stero-idogenesis and morphometric technique of stereology of some organella in granulosa cells,the results show that indomethacin suppressed ovulation but did not affect granulosa cells'conversion to lutein cells.The remarkable differences between the testing group and thecontrol are that collagenlysis was less observed in the tunica albuginea and theca externaof prevulatory follicles treated by indomethacin,and the surface epithelium of the folliclescontained many intact dense bodies without releasing their contents.The finding suggeststhat indomethacin acts mainly on the fibroblasts and surface epithelium in the follicular apexbefore ovulation,and this may have relationship with inhibition of the release of variousovulatory enzymes necessary to ovulation.