茯苓酸抗大鼠心脏移植急性排斥反应的实验研究

目的 探讨化合物茯苓酸对心脏移植急性排斥反应的抑制作用,为从中药中筛选出具有明确免疫抑制作用和低毒副作用的免疫抑制剂打下了良好的基础. 方法 以32只Wistar大鼠为供体,32只SD大鼠为受体,建立大鼠腹腔异位心脏移植模型,移植后的大鼠随机分为4组,每组8对.茯苓酸小剂量组:茯苓酸1 mg/(kg·d)橄榄油溶液灌胃;茯苓酸大剂量组:茯苓酸10 mg/(kg·d)橄榄油溶液灌胃;环孢菌素A(CsA)组: CsA 5 mg/(kg·d)橄榄油溶液灌胃;对照组:橄榄油溶液0.5 ml/(kg·d)灌胃.灌胃至观察终点.术后观察移植心存活时间,心脏跳动情况和心肌组织学改变,用酶联免疫吸附测定法(ELISA)检测血清白细胞介素2(IL-2)和γ干扰素(IFN-γ) 的含量.结果 茯苓酸大剂量组、茯苓酸小剂量组和CsA组移植心存活时间分别为24.90±0.99 d、15.50±1.60 d和26.80±0.88 d,均较对照组(6.10±1.10 d)显著延长(q=22.363, P=0.000; q=44.793, P=0.000; q=49.272, P=0.000).茯苓酸大剂量组、茯苓酸小剂量组和CsA组血清IL-2水平均低于对照组(q=14.483, P=0.000; q=3.705, P=0.000; q=21.418, P=0.000); 茯苓酸大剂量组低于茯苓酸小剂量组(q=10.778, P=0.000).茯苓酸大剂量组、茯苓酸小剂量组和CsA组血清IFN-γ水平均低于对照组(q=16.508, P=0.000; q=4.281, P=0.000; q=19.621, P=0.000);茯苓酸大剂量组低于小剂量组(q=14.975, P=0.000).术后第7 d移植心病理检查结果显示:茯苓酸小剂量组、茯苓酸大剂量组和CsA组心肌病理损害较对照组明显减轻. 结论 茯苓酸对心脏移植急性排斥反应具有明显的抑制作用.

Anti-acute Rejection Effect of Pachymic Acid in Heart Transplantation Rats

Objective To study the anti-acute rejection effect of Pachymic acid(PA) in heart transplantation rats,in order to select a new anti-rejection medicine with low side effect from traditional Chinese medicine.Methods We established the model by transplanting Wistar rats(32,donor) heart allografts into the abdomen of SD rats(32,receptor).The homologous heart-transplanted rats were then randomly divided into 4 groups with 16 rats in each group.Olive oil solution with PA 1 mg/(kg.d),PA 10 mg/(kg.d),Cyclosporine(C...