严重烧伤后早期大鼠肝脏细胞粘附分子1和白介素6的表达

目的：观察严重烧伤早期大鼠肝脏细胞粘附分子1(Intercellular adhesion molecule—1，ICAM—1)、白细胞介素6(Intedeukin6，IL—6)在不同时间点的表达及早期病理变化，为阐明烧伤早期肝脏损伤机制及为伤后早期损伤的防治打下基础。方法：(1)光镜和电镜观察大鼠在烧伤后早期(5min至72h)肝脏的病理变化。(2)免疫组织化学、原位杂交和图橡分析技术对伤后不同时间点肝脏ICAM—1进行染色和分析。(3)免疫组织化学、原位杂交和图像分析技术对伤后不同时间点肝脏IL—6进行染色和分析。结果：(1)伤后早期主要病理变化为出血、充血和水肿；电镜显示：肝脏毛细血管内皮细胞受损。(2)伤后30min，免疫组化和原位杂交显示ICAM—1、IL—6表达增强；伤后2h～24h明显增强，呈阳性或强阳性；伤后72h，表达逐渐减弱。结论：伤后早期肝脏的损伤可能与内皮细胞损伤有关，I-CAM—1、IL—6在伤后早期肝脏损害机制中占有重要地位。

Expression of hepatic intercellular adhesion molecule and interleukin 6 in rats after severe burns

Objective:To observe the expression and early pathologic changes of intercellular adhesion molecule 1(ICAM-1)and interleukin 6(IL-6)in rats after severe burus so as to demonstrate the mechanisms of early hepatic injuries and provide theoretic basis for prevention and treatment.Methods:Light and electron microscopes were employed to observe the early hepatic pathologic changes(5 minutes to 72 hours after burn).The expressions of ICAM-1 and IL-6 were observed by immunocytochemistry,in situ hybridization and image analysis.Results:Main early pathologic changes in liver were edema,hemorrhage and congestion.Injuries of the epithelium cells of capillary vessels were observed by electron microscope.Immunocytochemistry and in situ hybridization showed that ICAM-1 and IL-6 began to increase 30 minutes after burns,significantly increased 2 hours to 24 hours later, and gradually decreased 72 hours later.Conclusion:ICAM-1 and IL-6 may play an important role in early hepatic injuries,and the major target cell may be the endothelium cell.