脂蛋白(a)对鼠腹腔巨噬细胞释放一氧化氮的影响

为探讨脂蛋白 ( a)对巨噬细胞释放一氧化氮的影响 ,进一步了解脂蛋白 ( a)在动脉粥样硬化发生发展中的作用 ,将脂蛋白 ( a) (终浓度为 2 .5～ 40 mg/L )与鼠腹腔巨噬细胞共孵 ,检测上清液中乳酸脱氢酶活性、一氧化氮含量和细胞一氧化氮合酶活性。结果发现 ,在基础及脂多糖诱导状态下 ,细胞上清液中一氧化氮含量与脂蛋白 ( a)呈显著剂量依赖性降低 ,并呈时间效应。而细胞一氧化氮合酶活性则不受脂蛋白 ( a)刺激的影响。结果提示脂蛋白 ( a)对巨噬细胞释放 NO有明显抑制作用

The Effect of Lipoprotein(a) on Releasing of Nitric Oxide from Murine Peritoneal Macrophages

Aim To test the effect of lipoprotein(a) on releasing nitric oxide from macrophages in order to further investigate the role of lipoprotein(a) in the development of atherosclerosis.Methods Lipoprotein(a) with different concentration(range from 2.5 to 40 mg/L) was incubated with murine peritoneal macrophages stimulated by lipopolysacharride or without lipopolysacharride for 48h,and the activity of lactlate dehydrogenase and nitric oxide concentrations in cultured supernatants and the activity of nitric oxide synthase in macrophages were measured.Results Under both basic and stimulated conditions,lipoprotein(a) caused both dose dependent decrease in nitric oxide amounts in the supernatant,while had no effect on the NOS activity in macrophages.Conclusion These results suggest lipoprotein(a) may inhibit releasing nitric oxide from macrophages.