纤维支气管镜直视下肺刷落细胞端粒酶活性的检测

目的探讨纤维支气管镜下直接刷取肺脱落细胞中端粒酶活性在肺癌中的诊断价值。方法采用ＴＲＡＰ－ＰＣＲ－ＥＬＩＳＡ定量及ＴＲＡＰ－ＰＣＲ银染定性法,对５７例支气管肺刷落细胞进行端粒酶活性的分析。结果２３例肺癌患者患侧端粒酶活性水平明显高于其对侧肺部及肺炎组。与病理学相比,端粒酶在肺癌中的阳性检出率为９１．３％,明显高于肺脱落细胞涂片的阳性检出率（５２．２％）。同时还测得８３．３％的肺脱落细胞不典型增生者的端粒酶活性水平也显著增高。结论纤维支气管镜下直接刷取的肺脱落细胞中,端粒酶活性的测定作为诊断肺部恶性肿瘤有效而敏感的手段之一,可以提高肺癌的诊断率。

Detection of telomerase activity in bronchoscopic brush-off cells in lung cancer patients

Objective To investigate the diagnostic significance of the detection of telomerase activity in the exfoliated cells obtained from fiberobronchoscopic brushing. Methods The techniques of TRAP PCR ELISA and TRAP silver staining were employed to detecte telomerase activity in 57 cases with pulmonary diseases. Results Telomerase activity in the lesion side of lung cancer patients (N=23) was significantly higher than that in the contralateral side of the same patient( P <0.05), and in patients with pneumonia ( P <0.05). Positive rate of telomerase activity detection in lung cancer patients (91.3%) was significantely higher than that of cytological examination (52.3%, P <0.05). In 83.3% of the cases examined, exfoliated cells showing cytological dysplasia were found to be telomerase activity positive. Conclusion Detection of telomerase activity in the exfoliated cells obtained from fiberobronchoscopic brushing may be an effective and sensitive method in the diagnosis of pulmonary malignant diseases.