回心草单体成分对H2O2诱导心肌细胞损伤的保护作用研究

目的 建立过氧化氢（H202）诱导心肌细胞损伤模型，并在此模型上研究胡椒碱、胡椒酸甲酯、咖啡酸甲酯、尿嘧啶苷对心肌细胞损伤的保护作用。方法 用H202诱导造成原代培养的心肌细胞损伤模型，将单体化合物分为3个剂量组，作用于正常和损伤的心肌细胞，利用全自动生化分析仪对细胞悬液中乳酸脱氢酶（LDH）和肌酸激酶同功酶（CK-MB）含量进行测定。结果 回心草各单体成分对正常心肌细胞无明显影响。胡椒碱和胡椒酸甲酯各剂量组作用的心肌细胞悬液中LDH和CK-MB值与模型组对照有非常显著性差异（P＜0．01），咖啡酸甲酯对CK-MB有非常显著性差异（P＜0．01），尿嘧啶苷无显著性差异（P＞0．05）。结论 对H202诱导的心肌细胞损伤，胡椒酸甲酯和胡椒碱具有明显的保护作用，咖啡酸甲酯具有部分保护作用，尿嘧啶苷无保护作用。

The Secondary Metabolites from Rhodobryum Roseum (Hedw) Limpr.and Their Protective Effect on Injuried Cardiomyocytes Induced By H2O2

Aim To study the protective effect of peperine,methyl piperate,caffeic acid methyl ester and uracil glucoside from Rhodobryum roseum (Hedw) Limpr.on injuried cardiomyocytes induced by H_2O_2.Method Neonatal cardiomyocytes were cultured and cytotoxicity in cultured cardiomyocytes was induced by H_2O_2.Injuried and healthy cardiomyocytes were dealed by compounds.LDH and CK-MB activity in medium was assayed by automatic biochemistry analyzer.Result The compounds showed no significant effect on healthy cardiomyocytes.LDH and CK-MB activity in medium dealed by peperine,methyl piperate and CK-MB activity in medium dealed by caffeic acid methyl ester were significantly different from blank while those dealed by uracil glucoside was similarity.Conclusion Piperate and methyl piperate exhibited a good protecting effect on cardiac myocytes while uridine had not activity.Further biotest on cardiac myocytes with caffeic acid methyl easter was in progress.