| 透骨消痛颗粒对BMSCs向软骨细胞分化的影响 |
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| 引用本文： | 刘献祥,李明波,陈文列,陈鹏,吴追乐.透骨消痛颗粒对BMSCs向软骨细胞分化的影响[J].福建中医学院学报,2009,19(4):33-37. |
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| 作者姓名： | 刘献祥 李明波 陈文列 陈鹏 吴追乐 |
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| 作者单位： | 1. 福建中医学院中西医结合研究院,福建,福州,350108
2. 福建医科大学附属第一医院,福建,福州,350005 |
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| 基金项目： | 福建省自然科学基金，国家自然科学基金 |
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| 摘 要： | 目的 探讨透骨消痛颗粒舍药血清诱导骨髓间充质干细胞(BMSCs)向软骨细胞分化的分子生物学机制. 方法 从5只4周龄SD大鼠骨髓中分离出BMSCs,体外传代培养,取第3代BMSCs分为空白对照组、软骨诱导剂组及透骨消痛颗粒组,进行培养1、2周后,通过透射电镜、RT-PCR等实验技术,观察各组对BMSCs诱导成软骨细胞的影响. 结果 透射电镜观察可见软骨表型化的BMSCs多数为近椭圆形,表面突起多,细胞核大,粗面内质网丰富,内质网池扩张明显,高尔基体丰富.在TGF-β3等诱导因子作用下,透骨消痛颗粒舍药血清可以促进Sox9 mRNA、Cbfa1mRNA在细胞分化过程中的表达,与软骨诱导剂组相比表达量有显著性差异(P<0.05). 结论 透骨消痛颗粒含药血清可促进BMSCs向软骨细胞转化,改善软骨组织的质量,延缓软骨的退化.
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| 关 键 词： | 骨髓间充质干细胞 透骨消痛颗粒 软骨细胞 |
Effects of Tougu Xiaotong Granule on Differentiation of BMSCs into Chondrocytes |
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| LIU Xian-xiang,LI Ming-bo,CHEN Wen-lie,et al.Effects of Tougu Xiaotong Granule on Differentiation of BMSCs into Chondrocytes[J].Journal of Fujian College of Traditional Chinese Medicine,2009,19(4):33-37. |
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| Authors: | LIU Xian-xiang LI Ming-bo CHEN Wen-lie |
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| Institution: | Fujian Academy of Integrative Medicine;FUTCM;Fuzhou;Fujian 350108;China |
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| Abstract: | Objective To explore the molecular biological mechanism of differentiation of bone marrow stromal cells(BMSCs) into chondrocytes induced by Tougu Xiaotong granule drug-containing serum.Methods BMSCs were isolated from bone marrow of five 4-week-old SD rats and passage cultured in vitro,and the third generation of BMSCs were divided into control group,cartilage inducer group and Tougu Xiao-tong granule group,then after one or two weeks,the effects of each group on BMSCs,which were induced into chondrocytes,w... |
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| Keywords: | bone marrow stromal cells Tougu Xiaotong granule chondrocyte |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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