苦参碱对人肝癌细胞及正常肝细胞增殖和粘附功能影响的比较

目的观察苦参碱对人肝癌细胞BEL-7404及正常肝细胞QSG-7701增殖和粘附能力的影响。方法采用MTT法测定苦参碱对BEL-7404和QSG-7701的增殖;倒置相差显微镜观察细胞形态学变化;粘附实验检测细胞粘附率。结果苦参碱1.0～4.0g/L能有效抑制BEL-7404细胞的增殖。0.5～1.0g/L浓度的苦参碱能有效地诱导BEL-7404分化。当苦参碱的浓度低于0.5g/L时,其对QSG-7701细胞的增殖有一定的促进作用。0.25、0.5、0.75、1.0g/L苦参碱处理72h后,BEL-7404细胞黏附抑制率分别为18.6%、24.4%、30.3%、44.7%,与对照组比较差异均有显著性（P〈0.01）;而QSG-7701细胞黏附抑制率分别为1.7%、3.5%、6.1%、37.8%,只有1.0g/L浓度的苦参碱与对照组比较有差异显著性（P〈0.01）。结论苦参碱能有效抑制肝癌细胞BEL-7404的增殖,且抑制作用具有时间、剂量依赖性;低浓度能有效地诱导肝癌细胞分化;高浓度的苦参碱有较强的抑制人正常肝细胞增殖作用,低浓度具有一定的生长刺激作用;苦参碱抑制BEL-7404细胞粘附。

Effect of Matrine on cultured human hepatocytes and hepatoma carcinoma cells in proliferation and adhesion

Objective To investigate the inhibition effect of Matrine on the proliferation and adhersion of hepatoma carcinoma cells BEL-7404 and cultured human hepatocytes QSG-7701.Methods MTT assay was used to determine the proliferation inhibition by Matrine to BEL-7404 cell and QSG-7701 cell; to observe the rnorphocytology by inverted phase contrast microscope;Adhesive ratio was detected by adhesion assay. Results Matrine （1.0- 2.0g/L ）can inhibit the proliferation of BEL-7404 cells. Matrine （0.5- 1.0g/L ） can induce differentiation of BEL-7404 cells.When the matrine concentrations was less than 0.5g/L,it could promote the proliferation of QSG-7701 cells.After BEL-7404 cells were treated with Matrine of 0.25,0.5,0.75,1.0g/L for 72h respectively,adhesive ratios were18.6%,24.4%,30.3%,44.7% respectively, Compared with that of the control group, the adhesive ratio of all groups was significantly different（P〈0.01）; After QSG-7701 cells were treated with Matrine of 0.25,0.5,0.75,1.0g/L for 72h respectively, adhesive ratios were 1.7% ,3.5% ,6.1%,37.8% respectively,Compared with that of the control group, the adhesive ratio of 1.0 g/L group was signifi- cantly different （P〈0.01）. Conclusion Matrine has a significant inhibiting effect on human hepatocareinoma cell proliferation in a dose-and time-dependent manner;Low concentrations can effectively induce hepatoma cell differentiation;High concentrations of Matrine can strongly prohibit human liver cell proliferation, and low concentration has a certain growth-stimulating effect; Matrine inhibit BEL-7404 cell adhesion.