磷酸钠对砷致细胞膜损伤保护作用

目的 探讨磷酸钠对三价砷所致细胞膜损伤的保护作用。方法 用NaAsO₂处理非洲绿猴肾上皮细胞(Vero)后,在培养液中加入不同剂量磷酸钠剂,采用分光光度法分别测定细胞膜Na⁺,K⁺-ATP酶活性,高效液相法测定细胞膜磷脂组分含量改变。结果 2.20mg/L剂量的砷暴露可明显抑制Vero细胞膜Na⁺,K⁺-ATP酶活性;高剂量磷酸钠(69.00 mg/L)干预后,Na⁺,K⁺-ATP酶活性为(0.761±0.067)U/(mgprot.h)高于砷染毒组(0.544±0.037)U/(mgprot.h)(P<0.05);69.00 mg/L磷酸钠组细胞膜磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸及神经鞘磷脂含量分别为(0.506±0.073),(0.101±0.237),(0.083±0.005)和(0.066±0.003)mg/mL,明显高于砷染毒组(P<0.05)。结论 一定剂量磷酸钠对砷染毒细胞的细胞膜Na⁺,K⁺-ATP酶活性及磷脂组分的改变具有保护作用。

Protective effect of trisodium phosphate on arsenic-induced membrane damage

Objective To research protective effects of trisodium phosphate on Vero cells exposed to NaAsO₂.Methods After treatment of NaAsO₂,the Vero cells were cultured in nutrient fluid with different dosages of trisodium phosphate.The activity of Na⁺,K⁺ ATP ase in Vero cell membrane were measured with spectrophotometric.The contents of membrane phospholipids in Vero cells were detected with high-performance liquid chroma to graphy (HPLC).Results NaAsO₂ (2.20 mg/L) inhibited the activity of Na⁺,K⁺ ATPase in Vero cell membrane.Trisodium phosphate at high dosag e (69.00 mg/L) increased the activity of Na⁺,K⁺ ATPase (0.761±0.067 μU prot/h) compared with that of the control group(0.544±0.037 μU prot/h,P < 0.05).Meanwhile,The contents of four main components of membrane phospholipids in Vero cells were decreased with NaAsO₂ (2.20 mg/L) exposure.Trisod iumphosphate (69 00 mg/L) increased the contents of membrane phospholipids.Phosphatidy lcholine,phosphatidy lethano-lamine,phosphatidy lserin,and sphingm yelin in Vero cells were 0.506±0.073,0.101±0.237,0.083±0.005,and 0.066±0.003,respectively,and significantly higher than those of the control group (P < 0.05 for all).Conclusion Trisodium phosphate intervention at a certain dose could protect cells exposed to NaAsO₂ from changes of Na⁺,K⁺ ATPase activity and phospholipds content of the membrane.