感觉神经损伤盐敏感性高血压大鼠氧化应激

目的 探讨感觉神经损伤性盐敏感性高血压大鼠的氧化应激反应。方法 28只雄性Wistar大鼠分成4组:对照组、高盐组、辣椒辣素组和辣椒辣素高盐组,出生后第1、2 d 2次皮下注射辣椒辣素(CAP)50 mg/kg,动物在哺乳期结束时,高盐组给予高盐饲料(含4%NaCl),制备感觉神经损伤性盐敏感性高血压大鼠,连续4周;检测大鼠尾收缩压、血浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力、一氧化氮(NO)含量和一氧化氮合酶(NOS)活力。结果 与对照、高盐、辣椒辣素组比较,辣椒辣素高盐组大鼠尾收缩压[(151.43±4.20)mm Hg]、MDA含量[(2.14±0.51)nmol/mL]明显升高,差异有统计学意义(P<0.05);辣椒辣素高盐组SOD活力[(185.04±3.62)U/mL]、NO含量[(49.12±3.86)μmol/L]和NOS活力[(16.99±2.98)U/mL]明显降低(P<0.05)。结论 感觉神经损伤性盐敏感性高血压大鼠存在氧化应激反应。

Oxidative stress of salt-sensitive hypertension induced by sensory denervation

Objective To explore oxidative stress of salt-sensitive hypertension induced by sensory denervation.Methods Male Wistar rats were given 50 mg/kg capsaic in subcutaneously on the 1st and 2nd day of life.The control rats were treated with vehicle.After weanling period,the rats in treated and control groups were subdivided into 2 groups,respectively,and fed different saltdiets for 4 weeks(control,high sodium diet,capsaicin,and capsaicin plus high sodium diet).The model of salt sensitive hyper tension induced by sensory denervation was established.The plasm a content of malondialdehyde (MDA) and nitrogen monoxide(NO),the plasm a activity of super oxide dismutase(SOD) and nitric oxide synthase(NOS) were tested.Results The tail-cuff systolic blood pressure (mmHg) of capsaic in plus high sodium group increased significantly (151.43±4.20).The content of MDA were significantly higher in capsaicin plus high sodium group(2.14±0.51 nmol/L) than that of the control group,capsaicin group and high sodium group(P < 0.05 for all).The plasm a activity of SOD (185.04±3.62 U/m l) and NOS(16.99±2.98 U/ml),and the plasm a content of NO (49.12±3.86 mol/L) were all significanatly lower in capsaicin plus high sodium group than that of the control group and high sodium group (P < 0.01).The indexes also decreased in capsaic in plus high sodium group than in high sodium group(P < 0.05).Conclusion There is oxidative stress in the rats with salt-sensitive hypertension induced by sensory denervation.