高温和内毒素联合对RAW264.7细胞凋亡影响

目的 研究高温和内毒素复合因素对RAW264.7细胞凋亡的影响。方法 将RAW264.7细胞分成37℃空白(BLANK)组、37℃内毒素(LPS)组、40℃空白组和40℃内毒素组,置于含50%CO2细胞培养箱中,分别于3、24 h后取出,用流式细胞术检测细胞凋亡百分率,并对凋亡细胞生化标志性指标DNA片段进行检测。结果 37℃BLANK3 h组和24 h组细胞凋亡率极低,分别为(3.6±0.7)%和(4.6±1.9)%,同时37℃LPS 3 h组未见明显细胞凋亡现象,凋亡率为(2.8±0.6)%;而40℃LPS组凋亡情况较37℃LPS组严重,其中,40℃LPS 3 h组细胞凋亡率(6.7±0.6)%与37℃LPS 3 h组比较明显增高(P<0.05);40℃BLANK3 h组凋亡率与37℃BLANK3 h组比较虽有所增加,但差异无统计学意义(P>0.05);随着时间的延长,细胞凋亡情况明显加剧,37℃LPS 24 h组、40℃BLANK24 h组和40℃LPS 24 h组细胞凋亡率分别为(13.4±5.5)%、(21.2±2.8)%、(67.4±3.8)%,与同温度不同时间组比较,差异均有统计学意义(P<0.05)。结论 高温和内毒素对RAW264.7细胞具有损伤作用,在高温和内毒素作用下细胞会出现明显凋亡现象,且随着时间的延长,损伤程度逐渐加重,凋亡细胞数量大大增加。

Effects of high temperature combined with endotoxin on apoptosis of RAW264.7 cell

Objective To study the effect of high temperature combined with endotoxin on the apoptosis of RAW 264.7 cell.Methods The cultured RAW 264.7 cells in vitro were divided into 4 groups:normal temperature(37℃) blank,high temperature(40℃) blank,37℃+lipopoly saccharide(LPS),and 40℃+LPS guoup.After 3 and 24 hours culture under the condition of 95% O₂ and 5% CO₂,we detected the apoptoisis rate of RAW 264.7 cells with flow cytometry.Results Apoptosis rates were extremely low in 37℃ blank group(both 3hr and 24hr),as well as in 37℃+LPS group.The high temperature(40℃) groups,on the other hand,showed higher possibilities of cell apoptosis.Compared with 37℃+LPS goups,40℃+LPS group showed a much more obvious tendency of cell apoptosis(P < 0.05).But there was no significant difference(P > 0.05) between the apoptosis rate of 40℃ blank group and that of 37℃ group.As time passed by,the rate of cell apoptosis increased markedly (P < 0.05).Conclusion High temperature combined with endotoxin plays an importantrole in the apoptosis of RAW 264.7 cell.And the severity of cell apoptosis rises significantly with the extension of exposure tmie.