间歇性重度低氧对大鼠学习记忆影响

目的 建立不同暴露时间点大鼠间歇重度低氧模型,探讨间歇重度低氧对大鼠学习记忆功能的影响.方法 成年雄性W istar大鼠48只分为对照组和间歇性低氧组;采用低氧舱模拟5%间歇低氧环境.在间歇低氧2、4、6、8周采用Morris水迷宫检测学习记忆功能,苏木精-伊红(HE)染色观察海马区神经细胞形态变化.结果 与对照组比较,间歇性低氧组中神经细胞形态结构损伤明显,存活神经元密度(13.18±2.18)随低氧时间延长降低(P<0.05);低氧2、4、6、8周大鼠逃避潜伏期时间分别为(49.17±8.87)、(58.47±6.98)、(65.15±7.44)和(68.42±7.91)s,随低氧时间延长动物逃避潜伏期时间延长(P<0.5).结论 间歇性低氧可造成神经细胞损伤、学习记忆功能障碍,且随间歇性低氧时间延长而加重.

Effects of intermittent serious hypoxia on learning and memory function in rats

Objective To establish a hypoxia ammial model with differen texposed periods and severe intermittent hypoxia and to explore effects of severe interm ittent hypoxia on learning and memory function inrats.Methods Male Wistar rats(n=48) were randomly divided intochronic intermitten thypoxia group and control group.The 5% hypo xia models was made with hypoxia box.AT2, 4, 6, and 8 weeks after hypoxia, the morphologic changes of neuron were observed with HE staining.The learning and memory ability of the rats were assessed with Morris water maze.Results Compared with the control group, neuronal morphologic structure in the hypoxia group was damaged significantly.The survival neuronal density was decreased with prolonged hypo xia(13.18±2.18).The mean of escaping latency period was 49.17±8.87, 58.47±6.98, 65.15±7.44, and 68.42±7.91 seconds at 2, 4, 6, and 8 weeks in intermittent hypoxia group,which was decreased with prolonged hypoxia(P < 0.5).Conclusion Intermitten thypoxia can cause nervecell damage and learning memory dysfunction.The damage increases with the prolonged in termittent hypoxia.