辽宁省风疹野病毒基因特征分析

目的 了解辽宁省现阶段流行的风疹野病毒基因特征,为风疹的控制和消除提供科学依据。方法 用Vero/Slam细胞从2007-2008年采集的风疹暴发和散发患者标本中分离到22株风疹野病毒(Rubella Virus,RV),应用逆转录-聚合酶链反应(RT-PCR)针对RV分离株的E 1基因739个核苷酸片段进行PCR扩增,再对扩增产物进行核苷酸序列测定,与世界卫生组织(WHO)RV基因型参考株进行分子流行病学比较分析。结果 核苷酸和氨基酸同源性比对及构建亲缘关系树结果显示,22株RV分离株属1 E基因型,核苷酸和氨基酸同源性分别为97.6%～100.0%和99.1%～100.0%;与1 E基因型参考株序列的核苷酸和氨基酸同源性分别为97.0%～99.1%和99.1%～100.0%结论辽宁省分离的22株RV均为1 E基因型,并且可能是辽宁省现阶段的优势基因型。

Genetic characterization of wild-type rubella virus isolated in Liaoning province

Objective To analyze genetic characterization of rubella virusiso lated in Liaoning province and to provide scientific basis for rubella control and elmiination.Methods Vero/Slam cells were used for rubella virus isolation and 22 rubella viruses were isolated from the specmiens collected from rubella outbreaks and sporadic patients during 2007-2008.The fragments containing 739 nucleotides of E1 genes from 22 rubella virus isolate swere amplified with RT PCR method.The PCR products were sequenced and analyzed with WHO rubella reference strains down loaded from Genbank.Results The results of homology and phylogenetic analyses based on 739 nucleotides showed that 22 rubella virus strains belong to 1E geno type.Twenty two strains showed a nucleotide acids and amino acid smiilarity of 97.6% 100.0% and 99,1% 100,0%.Compared to the 1E reference strains,nucleotide acids and amino acid similarity were 97.0% 99.1% and 99.1% 100.0%.Conclusion The results suggest that 1E genotype of rubella virus is the predom inance geno type in Liaoning province.