耳蜗微循环与内淋巴电位同时观察记录的方法

耳蜗微循环在听觉生理中起着十分重要的作用,因此研究内耳供血不足引起的微循环改变,以及这种改变与听功能损伤之间的关系,有助于了解内耳循环生理及许多内耳疾病的发生机理。但是以往对耳蜗微循环的研究,多集中在观察微循环自身的改变上,忽略了微循环与听功能关系的研究。本文介绍了活体豚鼠耳蜗微循环与内淋巴电位同时观察记录的方法,将二者分析比较,并应用耳蜗缺血的实验模型观察了本方法的使用情况,取得满意的实验结果。本方法的建立为进一步研究听觉生理与微循环供血之关系及许多内耳疾病的发生机理奠定了基础。

The Technique for Measurement of Cochlear Microcirculation and Endocochlear Potential Simultaneously in Guinea Pigs

Cochlear microcirculation plays an important role in maintaining the normal functon of the auditory system. So observation of the microcirculation of the cochlea and the auditory function and the accessment of the relationship between the changes in cochlear microcirculation and auditory dysfunctions are valuable for understanding the mechanisms underlying some auditory dysfunctions induced by insufficient cochlear blood flow. In previous studies, however, major interests were directed toward the changes in cochlear blood flow itself, but much less attention was directed to the observation of cochlear function and cochlear microcirculation simultaneously. This paper intraduced the technique for measurement of cochlear microcirculation and endocochlear potential(EP) simultaneously and measured the changes in the microcirculation and endocohlear potential in the guinea pig with iscbemia of the cochlea. The current data indicate that this method can provides a dynamic quantitative assessment of cochlear microcirculation and EP.