肺心病患者细胞内游离钙-钙调蛋白活性的研究

目的：通过测定肺心病患者血小板内游离钙离子浓度和钙调蛋白活性，探讨和ＣａＭ活性变化在肺心病，缺氧性肺动脉高压形成机制中的作用。方法：用荧光指标剂Ｆｕｒａ－２／ＡＭ标记示踪法和磷酸二酯酶法测定１９例肺心病急性发作期患者和２０例健康者血小板（Ｃａ＾２＋）＾ｉ，ＣａＭ活性。

Platelet Free Calcium-calmodulin Content in Cor Pulmonale

Objective: To investigate the effect of platelet free calcium (Ca 2 ]i) and calmodulin (CaM) activity on cor pulmonale and pulmonary hypertension caused by hypoxia. Methods: We assayed the consentration of platelet Ca 2 ]i and the activity of calmodulin CaM respectively of 19 cor pulmonale pateints in their acute stage and of 20 healthy persons, using methods of Fura 2/AM trace labelling with fluorescence and of phosphodiesterase. Results: The concentration of Ca 2 ]i in cor pulmonale group is higher than that of normal group significantly. In cor pulmonale group, the activity of CaM correlated positively with Ca 2 ]i, negatively with arterial blood oxygen pressure (PaO 2), and positively with arterial blood carbon dioxide pressure (PaCO 2) significantly. Conclusion: The activity of Ca 2 ]i CaM increased in acute stage of cor pulmonale, anoxia and the retension of carbon dioxide are responsible for Ca 2 ]i increasing.