| 原发性肝细胞癌1号染色体杂合子丢失的初步研究 |
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| 引用本文： | Shao J,Li H,Liew CT,Wu Q,Liang X,Hou J. 原发性肝细胞癌1号染色体杂合子丢失的初步研究[J]. 中华病理学杂志, 1999, 0(1): 28-30 |
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| 作者姓名： | Shao J Li H Liew CT Wu Q Liang X Hou J |
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| 作者单位： | [1]广州中山医科大学肿瘤研究所病理科 [2]香港中文学医学院病理解剖及细胞病理学系 |
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| 摘 要： | 目的 分析原发性肝细胞癌（ＨＣＣ）１号染色体短臂（１ｐ）上１０个位点等位基因杂合子丢失（ＬＯＨ）,以期寻找１ｐ上可能存在的与ＨＣＣ发生有关的肿瘤抑制基因的缺失区域。方法 用聚合酶链反应（ＰＣＲ）方法分析３８例ＨＣＣ的１号染色体短臂１０个位点微卫星多态性标记的ＬＯＨ。所有患者均做ＨＢＶ５项检测和ＨＣＶ血清抗体的ＥＬＩＳＡ法检测。结果 在８４．２％（３２／３８例）的ＨＣＣ组织中检出染色体１ｐ片段的ＬＯ
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| 关 键 词： | 肝肿瘤 染色体畸变 肝细胞癌 杂合子丢失 |
A preliminary study of loss of heterozygosity on chromosome 1p in primary hepatocellular carcinoma |
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| Shao J,Li H,Liew C T,Wu Q,Liang X,Hou J. A preliminary study of loss of heterozygosity on chromosome 1p in primary hepatocellular carcinoma[J]. Chinese Journal of Pathology, 1999, 0(1): 28-30 |
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| Authors: | Shao J Li H Liew C T Wu Q Liang X Hou J |
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| Affiliation: | Department of Pathology, Cancer Center, Sun Yat-Set University of Medical Sciences, Guangzhou 510060. |
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| Abstract: | Objective Ten (10) loci on chromosome 1p were analyzed to detect LOH in 38 cases of hepatocellular carcinomas (HCC)in order to locate the deletion area and the possible deleted tumor suppressor gene (TSG) in HCC. Methods PCR based microsatellite instability analysis were used to detect LOH on chromosome 1p31 and 1p35 p36 in HCC. Results LOH were detected on chromosome 1p in 84.2% of HCC. High frequency of LOH (>40%) were occurred at locus D1S186 on 1p31, locus D1S482 on 1p35 and loci D1S243, D1S160, D1S165 and D1S170 on 1p36. Conclusion LOH occurred mostly on chromosome 1p31 and 1p35 p36 in HCC. There may be more than two TSGs which associated with development of HCC on chromosome 1p31 and 1p35 p36. |
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| Keywords: | Liver neoplasms Chromosomes human pair 1 Heterozygote Genes suppressor tumor |
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