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Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism |
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| Authors: | T. Brüning J. Abel B. Koch K. Lorenzen V. Harth S. Donat A. Sachinidis H. Vetter H. M. Bolt Y. Ko |
| Affiliation: | Institut für Arbeitsphysiologie an der Universit?t Dortmund, Ardeystrasse 67, 44139 Dortmund, Germany, DE Medizinisches Institut für Umwelthygiene an der Heinrich-Heine Universit?t, Auf'm Hennekamp 50, 40225 Düsseldorf, Germany, DE Medizinische Universit?ts-Poliklinik Bonn, Wilhelmstrasse 35-37, 53111 Bonn, Germany e-mail: yonko@uni-bonn.de Tel.: 0228/287-2263; Fax: 0228/287-2632, DE |
| Abstract: | The aim of the present study was to develop and validate a rapid assay for genotyping of CYP1B1 codon 432-polymorphism. The described method is a single tube assay and combines both rapid-cycle polymerase chain reaction (PCR) with real-time monitoring by amplification and generation of the melting profiles of an allele-specific fluorescent probe. With this method 300 samples were analysed from healthy, unrelated Germans. Genotype frequency determined for the mutated allele CYP1B1*2 was 0.40. The results show that genotyping of CYP1B1 codon 432-polymorphism with a real-time fluorescence PCR method is a rapid and reliable assay for the analysis of large numbers of samples. Received: 1 July 1999 / Accepted: 18 August 1999 |
| Keywords: | CYP1B1 Light-Cycler |
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