重组病毒巨噬细胞炎性蛋白在大鼠体内的药动学及组织分布

 目的了解重组病毒巨噬细胞炎性蛋白(vMIP)在SD大鼠体内的药动学及组织分布。方法对vMIP进行碘标、分离纯化和鉴定,然后利用其进行药动学实验。采用同位素示踪法测量vMIP在SD大鼠体内的药动学,并检测其在大鼠组织中的分布。结果60,240μg·kg^-1 2个剂量组(静脉注射给药)的主要药动学参数分别为ρ_max(249.4±84.50),(887.01±204.22)μg·L^-1;t_1/2β(1.5±0.28),(2.65±0.85)h;AUC_0-∞(136.0±40.70),(493.84±49.42)μg·h·L^-1。t_max10.0min。结论vMIP进入大鼠体内主要分布在肝和肺,在体内消除较快,其动力学特征符合一级吸收二室开放模型。

Pharmacokinetics and Tissue Distribution of Recombinant vMIP in Rats

OBJECTIVE To investigate the pharmacokinetics and tissue distribution of recombinant vMIP in SD rats.METHODS The vMIP was prepared by radioiodine label,then separated,purified and identified with the ¹²⁵I-vMIP.The ¹²⁵I-vMIP was determined and tissue distribution of vMIP was discribed in SD rats.RESULTS The main pharmacokinetic parameters of the low,middle(60 μg·kg^-1) and high dosage(240 μg·kg^-1) groups(after intra-venous administration) were as follow:ρ_max(249.4±84.50),(887.01±204.22) μg·L^-1;t_1/2β(1.5±0.28),(2.65±0.85) h ;AUC_0-∞(136.0±40.70),(493.84±49.42) μg·h·L^-1.t_max10.0 min.CONCLUSION vMIP was distributed mostly in lung and liver.The vMIP was eliminated rapidly in rats.The pharmacokinetic profile was described by a 2-compartment open model with the first order absorption.