实验性多发性肌炎动物模型制作的研究

目的 研究实验性多发性肌炎动物模型的制作方法。方法 利用家兔骨骼肌匀浆加弗氏完全佐剂多次免疫豚鼠制成实验性多发性肌炎模型,观察其症状表现、肌肉病理及肌酶谱的变化。结果 模型组豚鼠于免疫注射第5周开始有活动减少、倦怠嗜卧、食欲及体重明显下降等表现;肌肉病理活检可见模型组肌横纹模糊、消失,肌纤维排列紊乱或变性坏死,肌间结缔组织增生;肌酶谱中肌酸磷酸激酶(CK)、乳酸脱氢酶(LDH)和谷草转氨酶(AST)与对照组比较,均明显升高(P<0.01)。结论 提示本研究造模方法可作为研究人类多发性肌炎的一个重要手段,可为阐明其发病机理及治疗提供科学的理论依据。

The establishment of animal experimental polymyositis model

Objective To study the method of the establishment of an animal experimental polymyositis model . Methods An animal model of experimental polymyositis was produced in guinea - pigs by rabbit skeletal muscle homogenates added to Freund's complete adjuvant (FCA). It was observated that animals showed, symptom, body weights, pathology of the animals' skeletal muscles and the serum enzymes (CK, LDH, AST) .Results The guinea-pigs of model group had the symptoms such as activity reducing, acceded, indulge in lying and loss weighting after 5 weeks of immune injection. Pathology of the muscles exhibited that the arrangement of muscle fibers was disordered or necrosised, the connective tissue among muscles was hyperplastic, the serum enzymes (CK、LDH、AST) increased significantly compare with those of the control group( P < 0.01). Conclusion The results suggested that this method of establishing an animal model of experimental polymyositis is feasible, dependable and successful.