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| 单个核细胞和单核源性巨噬细胞生长特性的体外实验研究 | |
| 引用本文： | 张蔚然,张蓓,杨瑛,陈敏,蒲丹. 单个核细胞和单核源性巨噬细胞生长特性的体外实验研究[J]. 西部医学, 2011, 23(2): 220-222. DOI: 10.3969/j.issn.1672-3511.2011.02.007 |
| 作者姓名： | 张蔚然 张蓓 杨瑛 陈敏 蒲丹 |
| 作者单位： | 1. 四川省中西医结合医院急诊科,四川,成都,610041 2. 成都市第二人民医院神经内科,四川,成都,610017 3. 四川大学华西医院体检中心,四川,成都,610041 |
| 摘 要： | 目的研究人外周血单个核细胞、单核源性巨噬细胞在体外培养环境中的生长曲线情况。方法以密度梯度离心法和贴壁法分离、纯化正常人的外周血单个核细胞。以佛波醇酯促进单核源性巨噬细胞的分化成熟。用MTT法检测单个核细胞、单核源性巨噬细胞的生长曲线。采用单因素方差分析进行统计学检验。结果单个核细胞接种第1天的细胞数量明显多于第3、5、6、7天（P〈0.05）,但接种后第2~7天的细胞数量相对稳定（P〉0.05）。单核源性巨噬细胞分化成熟后第1~6天的OD,细胞数量较稳定。但第7天的细胞数量明显少于前6天（P〈0.05）。结论体外培养第2~7天的外周血单个核细胞处于生长平台期。体外培养的单核源性巨噬细胞在分化成熟后的第1~6天处于生长平台期。 |
| 关 键 词： | 单个核细胞 单核源性巨噬细胞 生长曲线 |
Growth feature of mononuclear cell and monocyte-derived macrophage in vitro |
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| ZHANG Wei-ran,ZHANG Pei,YANG Ying,et al. Growth feature of mononuclear cell and monocyte-derived macrophage in vitro[J]. , 2011, 23(2): 220-222. DOI: 10.3969/j.issn.1672-3511.2011.02.007 | |
| Authors: | ZHANG Wei-ran ZHANG Pei YANG Ying et al |
| Affiliation: | ZHANG Wei-ran1,ZHANG Pei2,YANG Ying3,et al(1.Department of Emergency,Sichuan Hospital of Integrated Traditional and Western Medicine,Chengdu 610041,2.Department of Neurology,The Second People Hospital of Chengdu,Chengdu 610017,China,3.Management Department of Medical Quality,West China Hospital of Sichuan University,China) |
| Abstract: | Objective To explore the growth curve of mononuclear cells or monocyte-derived macrophage in vitro.Methods Ficoll gradient centrifugation was used to obtain mononuclear cells and remove non-adherent lymphocytes.Mononuclear cells were cultured with PMA(100nmol/L) to obtain monocyte-derived macrophage.Cellular growth curve was measured by MTT tests.Results The quantity of mononuclear cells was stable from 2th to 7th day after inoculation.The quantity of monocyte-derived macrophage was stable from 1th to 6th d... |
| Keywords: | Mononuclear cell Monocyte-derived macrophage Growth curve |
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