| 釉基质蛋白对人牙周膜成纤维细胞矿化相关蛋白的影响 |
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| 引用本文： | 黄晓山,刘兰宁,郭利明. 釉基质蛋白对人牙周膜成纤维细胞矿化相关蛋白的影响[J]. 西部医学, 2011, 23(2): 227-229. DOI: 10.3969/j.issn.1672-3511.2011.02.009 |
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| 作者姓名： | 黄晓山 刘兰宁 郭利明 |
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| 作者单位： | 1. 成都医学院第一附属医院,四川,成都,610500
2. 南方医科大学中西医结合门诊部 |
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| 摘 要： | 目的通过检测釉基质蛋白（Enamel matrix proteins,EMPs）作用于体外培养牙周膜成纤维细胞,对细胞骨涎蛋白、骨桥蛋白、骨粘连素、牙骨质附着蛋白（Cementum attachment protein,CAP）表达及碱性磷酸酶（alkaline phosphatase,ALP）活性的影响,探讨EMPs促进牙周组织再生的作用机制。方法酶消化法获得人牙周膜成纤维细胞（Human periodontal ligament fibroblast,hPDLF）用100mg/L的EMPs诱导,镜下逐日观察细胞形态变化,在3天、7天两个时间点利用免疫组化方法和图像分析技术检测和分析诱导条件作用后细胞骨涎蛋白、骨桥蛋白、骨粘连素表达的影响,检测细胞牙骨质附着蛋白的表达。利用ALP法检测诱导后细胞成骨活性的变化。结果 100mg/L的EMPs对hPDLF具有上调骨涎蛋白、骨桥蛋白、骨粘连素的作用,随时间延长这种促进作用愈明显;EMPs作用hPDLF 3天即表达牙骨质附着蛋白;EMPs可促进hPDLF碱性磷酸酶活性的增强。结论 EMPs在一定浓度下可促使hPDLF向成骨、成牙骨质样细胞分化。
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| 关 键 词： | 细胞培养 人牙周膜成纤维细胞 釉基质蛋白 |
The effects of enamel matrix proteins on the synthesis of non-collagenous proteins of periodontal ligament cells in vitro |
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| HUANG Xiao-shan,LIU Lan-ning. The effects of enamel matrix proteins on the synthesis of non-collagenous proteins of periodontal ligament cells in vitro[J]. , 2011, 23(2): 227-229. DOI: 10.3969/j.issn.1672-3511.2011.02.009 |
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| Authors: | HUANG Xiao-shan LIU Lan-ning |
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| Affiliation: | HUANG Xiao-shan,LIU Lan-ning(Department of Stomatolog,The First Affiliated Hospital of Chengdu Medical College,Chengdu 610500) |
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| Abstract: | Objective To investigate the biological effects of enamel matrix proteins on cultured human periodontal ligament fibroblasts(hPDLF) and explore the possibility of its accelerating periodontal regeneration.Methods hPDLF was dissected and cultured by digestion with bacterial collagenase.The hPDLF was treated with 100mg/L EMPs.The cells' shape was observed by microscope everyday.The immunohistochemistry and image analyses methods were used to determine the expression of non-collagenous proteins and cementum at... |
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| Keywords: | Cell culture Human periodontal ligament fibroblast Enamel matrix proteins |
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