| JAK2/STAT3通过上调HSP70蛋白介导TTX心肌保护作用 |
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| 引用本文： | 刘胜中,甘崇志,杨双强. JAK2/STAT3通过上调HSP70蛋白介导TTX心肌保护作用[J]. 西部医学, 2011, 23(3): 415-418. DOI: 10.3969/j.issn.1672-3511.2011.03.005 |
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| 作者姓名： | 刘胜中 甘崇志 杨双强 |
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| 作者单位： | 1. 四川省医学科学院·四川省人民医院胸心外科,四川,成都,610072
2. 重庆医科大学附属第一医院胸心外科,重庆,400016 |
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| 摘 要： | 目的研究JAK2/STAT3信号通路在河豚毒素（tetrodotoxin,TTX）心脏停搏液心肌保护中的作用。方法 24只Wistar大鼠随机均分为对照组、TTX组和TTX＋AG 490组,每组8只。对照组：开胸取左心室肌作为缺血前对照。TTX组：建立离体心脏Langendorff-Neely灌注模型,预灌注K-H缓冲液30min后,灌注4℃TTX心脏停搏液,停搏心脏并低温维持60min,复灌K-H缓冲液60min后,取左心室肌备用。TTX＋AG490组：操作同TTX组,但预灌和复灌时均在K-H缓冲液中加入JAK2抑制剂AG490（5μmol/L）。采用免疫组化法测定心肌组织中p-STAT3（磷酸化STAT3）和HSP 70表达量（protein expression index,PEI）,TUNEL检测心肌细胞凋亡指数（apoptosis index,AI）,比较组间的变化。结果与对照组相比,TTX组和TTX＋AG490组心肌组织中p-STAT3和HSP 70的表达量均明显增加（P〈0.05）;予以AG490处理后,p-STAT3和HSP 70的表达量较TTX组明显下降（P〈0.05）。HSP 70蛋白表达与p-STAT3蛋白表达呈正相关（r=0.826,P〈0.05）。经历缺血再灌注后,TTX组和TTX＋AG490组心肌细胞AI明显高于对照组（P〈0.05）;与TTX组相比,TTX＋AG490组AI显著增加（P〈0.05）。结论 JAK2/STAT3信号通路通过上调HSP 70的表达,调动心脏内源性保护机制,介导TTX心脏停搏液对缺血心肌的保护作用。
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| 关 键 词： | 信号转导及转录激活因子3 热休克蛋白70 凋亡 河豚毒素 心肌保护 |
JAK2/STAT3 pathway mediates myocardial protection of tetrodotoxin cardiaoplegia in rat hearts via upregulating the protein expression of HSP 70 |
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| LIU Sheng-zhong,GAN Chong-zhi,YANG Shuang-qiang. JAK2/STAT3 pathway mediates myocardial protection of tetrodotoxin cardiaoplegia in rat hearts via upregulating the protein expression of HSP 70[J]. , 2011, 23(3): 415-418. DOI: 10.3969/j.issn.1672-3511.2011.03.005 |
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| Authors: | LIU Sheng-zhong GAN Chong-zhi YANG Shuang-qiang |
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| Affiliation: | LIU Sheng-zhong1,GAN Chong-zhi1,YANG Shuang-qiang2(1.Department of Cardiothoracic Surgery,Sichuan Academy of Medical Sciences & Sichuan People's Hospital,Chengdu 610072,China,2.Department of Cardiothoracic Surgery,The First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To investigate the effect of JAK2/STAT3 pathway on myocardial protection of tetrodotoxin(TTX) cardioplegia in rat hearts.Methods 24 Wistar rats were randomly divided into Group Conteeerol,Group TTX and Group TTX+AG490(n=8).The left ventricular samples in Group Control were collected as pre-ischemia control through thoracotomy.After isolated heart Langendorff and Neely models were established the rat hearts in Group TTX were continuously perfused with Krebs-Henseleit(K-H) buffer solution for 30 min... |
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