| 人膀胱癌耐药细胞系T24/ADM的建立及其耐药机制 |
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| 引用本文： | 杨勇,姚启盛,陈从波,龚小新,黄力,王黎,刘正清.人膀胱癌耐药细胞系T24/ADM的建立及其耐药机制[J].西部医学,2011,23(2):213-215. |
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| 作者姓名： | 杨勇 姚启盛 陈从波 龚小新 黄力 王黎 刘正清 |
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| 作者单位： | 湖北医药学院附属太和医院泌尿外科,湖北,十堰,442000 |
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| 基金项目： | 湖北省教育厅科学技术研究计划重点项目(编号:D20082404) |
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| 摘 要： | 目的建立人膀胱癌耐药细胞系,对其耐药机制进行初步探讨。方法采用阿霉素浓度梯度递增诱导法,建立多药耐药细胞系T24/ADM。相差显微镜观察细胞,用MTT法检测该耐药细胞株对多种化疗药物的多药耐药性,应用RT-PCR和Western blot比较两种细胞P-GP、MRP和LRP的表达差异来探讨可能的耐药机制。结果建立了T24/ADM耐药细胞系,对ADM、VCR和VP16有交叉耐药,P-GP、MRP和LRP在耐药细胞系中表达升高。结论 T24/ADM具有明确的多药耐药性,其多药耐药相关基因表达升高是T24/ADM获得耐药的主要机制之一。
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| 关 键 词： | 膀胱肿瘤细胞 多药耐药性 阿霉素 |
Establishment of a multidrug resistance cell line of human bladder carcinoma T24/ADM and its multidrug resistant mechanisms |
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| YANG Yong,YAO Qi-sheng,CHEN Cong-bo,et al.Establishment of a multidrug resistance cell line of human bladder carcinoma T24/ADM and its multidrug resistant mechanisms[J].Medical Journal of West China,2011,23(2):213-215. |
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| Authors: | YANG Yong YAO Qi-sheng CHEN Cong-bo |
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| Institution: | YANG Yong,YAO Qi-sheng,CHEN Cong-bo,et al(Department of Urology,The Taihe Hospital of Yunyang Medical College,Shiyan 442000,Hubei,China) |
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| Abstract: | Objective To establish a multidrug resistance cell line of human bladder carcinoma and study the mechanism of multidrug resistance.Methods Human bladder carcinoma cell line T24 was induced to multidrug resistance cell line T24/ADM by intermittent administration of high dose ADM.The multidrug resistance was detected with MTT assay.The difference expressions of mdr-1 gene-coded P-glyeo protein(P-gp),multidrug resistance-associated protein(MRP),and LRP were examined with RT-PCR and Western blot.Results An adri... |
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| Keywords: | Human bladder carcinoma Multidrug resistance Adriamycin |
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