| 人血浆中孟鲁司特的LC-MS/MS法测定 |
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| 引用本文： | 刘钫,何丽娟,任进民,杨汉煜,赵曦.人血浆中孟鲁司特的LC-MS/MS法测定[J].中国医药工业杂志,2012,43(1):50-52. |
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| 作者姓名： | 刘钫 何丽娟 任进民 杨汉煜 赵曦 |
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| 作者单位： | 1. 石药集团中奇制药技术(石家庄)有限公司,河北石家庄,050051
2. 河北医科大学第二医院,河北石家庄,050000 |
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| 摘 要： | 建立了液相色谱-串联质谱法测定人血浆中的孟鲁司特.采用C18色谱柱,以辛伐他汀为内标,乙腈-水-甲酸(86∶14∶0.3)为流动相,ESI源正离子检测方式,多反应监测,监测离子对m/z 586.3→422.5(孟鲁司特)、m/z441.4→325.2(辛伐他汀).孟鲁司特在1～1 000 ng/ml浓度范围内线性关系良好.回收率为96.7％～110.6％,RSD小于7.0％.
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| 关 键 词： | 孟鲁司特 液相色谱-串联质谱 测定 |
Determination of Montelukast in Human Plasma by LC-MS/MS |
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| Authors: | LIU Fang HE Lijuan REN Jinmin YANG Hanyu ZHAO Xi |
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| Institution: | 1(1.Shijiazhuang Pharm Group Zhongqi Pharmaceutical Technology(Shijiazhuang) Co.,Ltd.,Shijiazhuang 050051; 2.The Second Hospital of Hebei Medical University,Shijiazhuang 050000) |
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| Abstract: | A LC-MS/MS method was established for the determination of montelukast in human plasma.A C18 column was used with the mobile phase of acetonitrile-water-formic acid(86︰14︰0.3) and simvastatin as the internal standard.Multiple reaction monitoring(MRM) mode was used with the transitions of m/z 586.3→422.5(montelukast) and m/z 441.4→325.2(simvastatin).The calibration curve was linear in the concentration range of 1-1 000 ng/ml.The recoveries were 96.7%-110.6%,with RSD less than 7.0%. |
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| Keywords: | montelukast LC-MS/MS determination |
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