预防用药对激素性股骨头骨细胞凋亡的影响

目的探讨应用药物预防激素性股骨头骨细胞凋亡的可能性。方法30只兔随机分A、B、C组:A组(对照组)同期肌注生理盐水;B组(激素组)给予糖皮质激素肌注;C组(治疗组)给予以上剂量糖皮质激素的同时,每日使用血塞通、脂必妥和阿仑膦酸钠,用末端原位标识(TUNEL)法检测骨细胞凋亡,电镜观察股骨头组织学变化。结果激素组电镜下骨细胞核体积缩小,染色质呈边聚现象,部分染色质更浓密,电子密度增加,结构模糊不清;激素和治疗组凋亡细胞数分别为(24.14±5.46)%和(8.12±3.23)%。结论血塞通、脂必妥和阿仑膦酸钠同时应用能抑制兔激素性股骨头细胞凋亡。

Preventive effect of drugs on apoptosis in hormonal necrosis of femoral head

Objective To explore constervation prevent of experimental on apoptosis of necrosis of femoral head. Metheds Thirty rabbits were divided randomly into 3 groups: group A and group B and group C . Rabbits in group A were injected with normal saline; rabbits in group B were treated with glucocorticoid; rabbits in group C were treated with glucocorticoid as that in group B, and meanwhile, orally received the panax notoginseng saponins, zhibituo and alenduonate for 8 weeks. Histopathological changes and apoptosis of femoral head were observed. Results Under electron microscope, group B showed a great deal of necrotic osteocytes with pyknotic nucleus, condensed chromatin and increased electron dense. Apoptosis in B group(24.14±5.46)% was more than that of C group(8.12±3.23)%. Conclusion The zhibituo, panax notoginseng saponins and alenduonate may inhibit apoptosis of hormonal necrosis of femoral head of rabbits.