不同转移潜能的人黑色素瘤细胞p53和MMPs基因突变的研究

目的探讨具有不同潜在转移能力的黑色素瘤细胞恶性表型的演进及其侵袭转移的机制。方法采用细胞培养，p53蛋白免疫组化，PCR－SSCP检测；酶活性分析，流式细胞术检测。结果人类黑色素瘤细胞中存在有p53基因的突变，瘤细胞表面671kDa层粘连蛋白受体（LN－R）的荧光阳性率和全部细胞的平均荧光强度大小顺序为：WM451＞WM983A＞WM1341B＞WM35。MMPs的产生情况：早期WM35不产生MMPs，WM1341B仅产生72kDa（MMP－2），不产生92kDa（MMP－9）；进展期WM983A和远处转移瘤株WM451均产生72kDa和92Kda。其侵袭、转移的潜能与其产生或诱导产生的MMPs的能力和其表面67kDaLN－R的表达水平密切相关。结论p53基因突变；MMPs的产生和细胞表面67kDaLN－R的高表达是人黑色素瘤细胞侵袭转移能力获得的关键因素。

Gene mutation of p53 and MMPs in human melanocarcinoma with various transfering potentials

Objective To research the progressive and the invasive trasfering mechanism of melanocarcinoma cells in malignant phenotype with varioustranefering. Methods To adopt cell culture; p53 protein immunohistochemical. PCR -SSCP examination; Zymography, FCM examination. Results There exists p53 gene mutation in human melanocarcoma, The fluorescence positive rate of 67KDa laminin - receptor(LN - R) in the surface of cancer cell and the order of the average fluorescence intensity are WM451 > WM983A >WM1341B>WM35. As to the situation in which MMPs is made, early WM35 produced no MMPs, WM1341B produced only 72KDa(MMP-2), but no 92KDa(MMP-9). Progressive WM983A and long term transfering tumorous cell strain WM451 both produced 72KDa and 92KDe. The invasive transfering potential was closely related to MMPs inducing ability and its extressing level of 67KDa LN-R in the surface. Conclusion Mutation of p53 gene and the produetion of MMPs and the high expression of 67KDa LN-R in cell surface play a key role in the acquisition of invasive transfering of human melanocarcinoma.