| 应用基质辅助激光解吸质谱的源内衰变及串联质谱技术测定重组人纽兰格林的C端序列 |
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| 引用本文： | 桑志红,刘炳玉,李萍,薛燕,杨松成,何昆. 应用基质辅助激光解吸质谱的源内衰变及串联质谱技术测定重组人纽兰格林的C端序列[J]. 军医进修学院学报, 2011, 32(9): 947-948. DOI: CNKI:11-3275/R.20110321.1755.003 |
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| 作者姓名： | 桑志红 刘炳玉 李萍 薛燕 杨松成 何昆 |
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| 作者单位： | 国家生物医学分析中心,北京,100850 |
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| 基金项目： | 科技部新药创制重大专项基金 |
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| 摘 要： | 目的测定重组人纽兰格林的C端序列。方法用基质辅助激光解吸电离飞行时间质谱（MALDI—TOF-MS）的源内裂解（ISD）及串联质谱技术（TOF—TOF，LIFT），测定重组蛋白纽兰格林的C端序列。结果用该方法鉴定了重组蛋白纽兰格林C端序列的10个氨基酸残基。结论与现有的C-端序列测定方法比较，该方法简便快捷，可适用于部分重组蛋白药物的质量控制。
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| 关 键 词： | 蛋白质组学 C端测序 基质辅助激光解吸电离飞行时间质谱的源内衰变 基质辅助激光解吸飞行时间串联质谱 |
Detection of recombinant human neuregulin C-terminal sequences by in-source-delay of MALDI-TOF-MS and LIFT of TOF-TOF-MS |
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| SANG Zhi-hong,LIU Bing-yu,LI Ping,XUE Yah,YANG Song-cheng,HE Kun. Detection of recombinant human neuregulin C-terminal sequences by in-source-delay of MALDI-TOF-MS and LIFT of TOF-TOF-MS[J]. Academic Journal of Pla Postgraduate Medical School, 2011, 32(9): 947-948. DOI: CNKI:11-3275/R.20110321.1755.003 |
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| Authors: | SANG Zhi-hong LIU Bing-yu LI Ping XUE Yah YANG Song-cheng HE Kun |
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| Affiliation: | National Center of Biomedical Analysis,Beijing 100850,China |
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| Abstract: | Objective To detect the recombinant human neuregulin（rhNRGL） C-terminal sequences. Methods Ten amino acid residues of rhNRGL C-terminal sequences were detected by in-source-delay（ISD） of MALDI-TOF-MS and LIFT of TOF-TOF-MS. Results The 10 amino acid residues of rhNRGL C-terminal sequences were detected. Conclusion This method is simpler and rapider than the commonly used method and can be applied to quality control of some recombinant proteins. |
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| Keywords: | Proteomics C-terminal sequencing IS D of MALDI-TOF-MS MALDI-TOF-TOF-MS |
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