乙型肝炎病毒大蛋白（LHBs）检测的临床价值

目的探讨乙型肝炎病毒大蛋白（LHBs）诊断乙型肝炎的意义。方法采用酶联免疫吸附测定（ELISA）检测乙型肝炎病毒大蛋白（LHBs）、乙肝病毒前S1（PreS1）,采用荧光定量PCR方法检测HBV DNA。结果 HBeAg阳性样本中乙型肝炎病毒大蛋白与HBV DNA的检出无显著性差异（χ2=2.46,P〉0.05）;乙肝病毒前S1与HBV DNA的检出有显著性差异（χ2=17.34,P〈0.01）;乙型肝炎病毒大蛋白吸光度值（OD值）与HBV DNA拷贝数相关系数r=0.912,P〈0.05。在HBeAg阴性样本中LHBs的吸光度均值和阳性率均呈线性增加,Pre S1的吸光度均值以及阳性率与HBV DNA拷贝数均不相关。结论乙型肝炎病毒大蛋白（LHBs）与HBV DNA有良好正相关,与乙肝前S1（Pre S1）相比,LHBs可更好反映临床乙肝病毒复制水平。

Hepatitis B virus large protein in diagnosis of Hepatitis B

Objective To study the role of hepatitis B virus large protein（HBVLP） in diagnosis of hepatitis B.Methods HBVLP and HBV PreS1 were detected by ELISA,and HBV DNA was detected by fluorescence quantitative polymerase chain reaction（PCR）.Results No significant difference was found in HBVLP and HBV DNA（χ2=2.46,P0.05） while a significant difference was observed in HBV PreS1 and HBV DNA（χ2=17.34,P0.01） in HBeAg positive samples.The OD value was closely correlated with the copies of HBV DNA（r=0.912,P0.05）.The mean OD value and positive rate for HBVLP were linearly increased in HBeAg negative samples.However,the mean OD value and positive rate for HBV PreS1 were not related the copies of HBV DNA.Conclusion HBV LP is positively related with HBV DNA and can show a higher HBV replication level than HBV PreS1.