两种创伤性深静脉血栓大鼠模型中炎症相关基因的表达研究

比较分析本课题组原创的急性、亚急性创伤性深静脉血栓形成大鼠模型中炎症相关基因的表达变化,探讨炎症在创伤性深静脉血栓形成中的意义。分别将急性、亚急性血栓形成模型各150只大鼠,随机分为正常组和模型组。急性血栓模型组采用直接钳夹股静脉 双后肢石膏固定,亚急性组采用定量击打双侧大腿 双后肢石膏固定的方式造模。根据深静脉血栓形成过程中不同的生物学状态,将实验动物分为正常对照、创伤即刻、血栓形成初始期、血栓形成高峰期、血栓消退、血栓不消退和血栓不形成7组。采用Gene-chip Rat Genome 4302.0芯片,动态测定股静脉RNA表达情况,运用基因芯片数据分析方法(倍数变化分析),筛选出差异性表达基因。重点分析和比较2种模型中炎症相关基因的表达变化。结果表明:两种模型中共有28个(急性模型中23个,亚急性模型中24个;其中2种模型中均出现差异性的基因19个;仅出现于一种模型中的8个)炎症相关基因呈现差异性表达,但两组的基因表达水平具有统计学差异(Z=-2.513,P=0.012<0.05)。说明炎症相关基因的变化与创伤性深静脉血栓形成密切相关,它可能通过影响内皮细胞的功能参与血栓形成。

The Study of Genes Expressions Related to Inflammation in Two Rat Models of Traumatic Deep Vein Thrombosis

Through comparing the expression changes of genes related to inflammation in the acute and subacute traumatic deep vein thrombosis(TDVT) rat models,to initially explore the significance of inflammation response in TDVT.In acute and subacute TDVT models,each 150 SD rats were divided into the control and experimental groups.Directly clamping femoral vein combined with hip spica fixation was used to establish acute TDVT model and quantitative beating on bilateral posterior limbs combined with hip spica cast fixation was used to establish subacute model.According to the different biological states,the experimental rats were divided into 7 subgroups: the control,the post-traumatic instant,the initial period of thrombosis,the crest-time of thrombosis,thrombi solution,thrombi insolution and no thrombosis subgroups.Genechip Rat Genome 430 2.0 gene chips were applied to detect the RNA expression of femoral vein dynamicly.Through the fold change analysis,the differential expression genes were selected for pathway analysis.The expression changes of genes related to inflammation in the two models were compared and analyzed.Results showed that: in the two mod els,28 genes in total related to inflammation response presented differential expression(23 in acute model,24 in subacute model,19 in both of them,8 only in one).But the difference of expression levels between the two groups had a statistical significance(Z=-2.513,P=0.012<0.05).The change of genes related to inflammation is correlated to TDVT,and which can through influence the function of endothelial cell to contribute to thrombosis.