| PFGE技术对一起副溶血性弧菌引起食物中毒的同源性研究 |
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| 引用本文： | 赵雪琴,商晓春,张睿.PFGE技术对一起副溶血性弧菌引起食物中毒的同源性研究[J].中国卫生检验杂志,2012(10):2388-2390. |
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| 作者姓名： | 赵雪琴 商晓春 张睿 |
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| 作者单位： | 浙江省杭州市下城区疾病预防控制中心 |
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| 摘 要： | 目的:了解一起食物中毒事件中副溶血性弧菌(VP)分离株的同源性。方法:按国标方法进行VP的分离与鉴定;对分离出的VP做血清分型及tdh、trh毒力基因检测;用脉冲场凝胶电泳(PFGE)技术进行基因分型。结果:从10例患者肛拭子和1份砧板涂抹物中分离到的11株VP,血清型均为O3:K6,tdh毒力基因均为阳性,PFGE分型图谱也完全相同。结论:这是一起因VP污染了食品加工环节而导致的食物中毒事件。
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| 关 键 词： | 脉冲场凝胶电泳(PFGE) 副溶血性弧菌 食物中毒 |
PFGE technology for study on homology of Vibrio parahaemolyticus strains in a food poisoning event |
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| ZHAO Xue-qin,SHANG Xiao-chun,ZHANG Rui.PFGE technology for study on homology of Vibrio parahaemolyticus strains in a food poisoning event[J].Chinses Journal of Health Laboratory Technology,2012(10):2388-2390. |
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| Authors: | ZHAO Xue-qin SHANG Xiao-chun ZHANG Rui |
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| Institution: | (Xiacheng District Center for Disease Control and Prevention,Hangzhou 310003,China) |
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| Abstract: | Objective:To understand the homology of the Vibrio parahaemolyticus(VP) strains isolated from a food poisoning.Methods:VP was isolated and identified according to the national standard.The isolated VP strains were carried out serotyping and virulence gene(tdh,trh) detection,then pulsed-field gel electrophoresis(PFGE) was used for genotyping.Results:11 VP strains with the serotype of O3:K6 were isolated from 10 patients and a chopping block.In this detection,all the VP isolates carried tdh virulence genes and had the same PFGE pattem.Conclusion:This is a food poisoning caused by VP pollution in the food processing link. |
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| Keywords: | Pulsed-field gel electrophoresis(PFGE) Vibrio parahaemolyticus Food poisoning |
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