RGDS-GG-KLAKLAKK多肽对人骨肉瘤细胞黏附迁移和侵袭力的影响

目的 研究多肽RGDS-GG-KLAKLAKK对体外培养人骨肉瘤细胞MG-63黏附迁移和侵袭力的影响,并探讨其作用机制.方法 将不同浓度(0、25、50、75、100μmoL/L)的多肽RGDSGG-KLAKLAKK与MG-63细胞混合培养,用比色法测量多肽RGDS-GG-KLAKLAKK对MG-63细胞黏附力的抑制作用,通过transwell小室侵袭模型检测多肽对MG-63细胞侵袭力的影响.观测多肽对骨肉瘤细胞自发肺转移模型抗肿瘤转移的影响.结果 MG-63骨肉瘤细胞对纤维连接蛋白(Fn)的黏附被多肽GDS-GG-KLAKLAKK对明显抑制(P<0.05).25、50、75、100 μmol/L的多肽RGDS-GG-KLAKLAKK对MG-63细胞侵袭力的抑制率分别为(18.3±2.9)%、(28.5±4.8)%、(40.4±5.6)%和(66.7±7.8)%,对比空白对照组(0μmol/L多肽)差异有统计学意义(P<0.05).多肽RGDS-GG-KLAKLAKK对MG-63骨肉瘤细胞浸润能力有明显抑制作用.MG-63骨肉瘤细胞接种于裸鼠后给予多肽RGDS-GG-KLAKLAKK治疗,与对照组比较,有明显的肺重减轻、肺转移灶减少(P<0.05).结论 多肽RGDS-GG-KLAKLAKK能够抑制人骨肉瘤MG-63细胞的体外黏附、侵袭和转移.

The anti-tumor adhesive and metabolic activities of RGDS-GG-KLAKLAKK peptides on the human osteosarcoma cells

Objective To investigate the effects of the peptides (RGDS-GG-KLAKLAKK)on the invasion and metastasis ability of human osteosarcoma cell(MG-63). Methods We co-cultivated the different concentrations of the peptides RGDS-GG-KLAKLAKK (concentration separately as 0,25,50,75, 100 μmol/L) with the MG-63 cell. Cell adhesion and cell invasion assay both in vitro and in vivo were used to investigate the inhibitory effects on MG-63 adhesion to fibronectin (Fn) coated surface. Trans well was used to assay the effect of RGDS-GG-KLAKLAKK on MG-63 cell invasive capability on reformed basement membrane. The effect of anti-metastatic peptides was observed in the MG-63 cell model that will automatically metastasize to lung. Results RGDS-GG-KLAKLAKK obviously suppressed the cell adhesion to fibronectin in a dose-dependent manners (P<0.05 ). After MG-63 cells were treated with peptides of 25,50,75,100 μmol/L for 48 h,the inhibitory ratios of invasion were (18.3 ±2.9)% ,(28.5 ±4.8)% , (40.4 ±5.6)% and (66. 7 ±7. 8)%,respectively (P<0. 05). MG-63 cells was implantated in node mice. RGDS-GG-KLAKLAKK treatment were done to each mice by 50,100 μmol/L,the lung metastatic nodule number and the weight of metastatic lung could be decreased significantly (P<0.05 ). Conclusion The pepetdes RGDS-GG-KLAKLAKK can suppress the invasive and metastatic activities of human osteosarcoma Cell line MG-63.