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| 外周血p53基因SNP检测与大肠癌早期诊断的关系 | |
| 引用本文： | 刘清,赖卓胜,王伟,张燕,周淼.外周血p53基因SNP检测与大肠癌早期诊断的关系[J].南方医科大学学报,2007,27(12):1939-1941. |
| 作者姓名： | 刘清 赖卓胜 王伟 张燕 周淼 |
| 作者单位： | 1. 广东省深圳市福田区梅林医院内科,广东,深圳,518049 2. 南方医科大学南方医院消化研究所,广东,广州,510515 3. 南方医科大学南方医院肿瘤研究所,广东,广州,510515 |
| 摘 要： | 目的分析大肠癌发生过程中p53基因突变的作用，评估外周血p53基因突变分析作为大肠癌高危对象筛查指标的实际应用价值。方法用LDR-PCR技术对100例大肠癌家系成员和100例健康人（对照组）的外周血p53基因第5-8外显子进行单核苷酸多态性（SNP）检测。结果大肠癌家系成员和对照组的外周血p53基因的突变率为分别为24％和0，差异有显著性（P〈0．05）。结论p53基因突变在大肠癌的发生过程中起重要作用，外周血p53基因SNP突变分析可望成为大肠癌高危对象的早期诊断手段。 |
| 关 键 词： | 大肠肿瘤 LDR-PCR SNP p53基因 |
| 文章编号： | 1673-4254(2007)12-1939-02 |
| 修稿时间： | 2007年4月15日 |
Peripheral blood p53 single nucleotide polymorphism analysis for early diagnosis of colorectal carcinoma |
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| LIU Qing,LAI Zhuo-sheng,WANG Wei,ZHANG Yan,ZHOU Miao.Peripheral blood p53 single nucleotide polymorphism analysis for early diagnosis of colorectal carcinoma[J].Journal of Southern Medical University,2007,27(12):1939-1941. | |
| Authors: | LIU Qing LAI Zhuo-sheng WANG Wei ZHANG Yan ZHOU Miao |
| Institution: | Department of Gastroenterology, Meilin Hospital, Shenzhen 518049, China. |
| Abstract: | Objective To evaluate the role of p53 gene mutation in colorectal carcinoma and assess the value of peripheral blood p53 single nucleotide polymorphism (SNP) in early diagnosis of colorectal carcinoma. Methods NSP in axons 5-8 of p53 gene was detected using ligase detection reaction-polymerase chain reaction (LDR-PCR) in the peripheral blood of 100 patients with colorectal cancer and 100 healthy subjects. Results The mutation rate of p53 gene was 24% (24/120) in colorectal carcinoma patients and 0% (0/120) in the healthy subjects (P<0.05). Conclusion p53 plays an important role in the carcinogenesis of colorectal carcinoma, and SNP analysis for p53 gene can be helpful in early diagnosis of colorectal carcinoma. |
| Keywords: | colorectal cancer ligase detection reaction-polymerase chain reaction single nucleotide polymorphism p53 gene |
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