单核细胞组织因子在急性缺血性脑卒中的动态变化

目的观察急性缺血性脑卒中(AIS)患者急性期及恢复期组织因子(TF)的变化。方法选择发病48 h内的AIS患者30例为AIS组,健康体检者61例为对照组。采用RT-PCR检测单核细胞TF mRNA;采用ELISA法检测血浆TF抗原。结果与对照组比较,AIS组急性期患者单核细胞TF mRNA(0.254±0.04 vs 0.312±0.09)和血浆TF抗原[(197.18±94.91)ng/L vs(276.64±106.01)ng/L]均明显增高,呈同步上升趋势(P<0.05);与急性期比较,AIS组恢复期单核细胞TF mRNA及血浆TF抗原呈同步下降,差异均有统计学意义(P<0.05)。AIS组恢复期血浆TF抗原与对照组比较差异无统计学意义(P>0.05)。结论 AIS患者发作期血液呈高凝状态,血栓形成风险增加;动态观察AIS患者单核细胞TF mRNA与血浆TF抗原含量,对了解疾病的发生、发展有辅助作用。

Dynamic change of tissue factor in monocytes of acute ischemic stroke patients

Objective To observe the change of tissue factor(TF)in monocyes of acute ischemic stroke(AIS) patients at acute phase and recovery stage.Methods Thirty AIS patients within 48 h after its attack served as an AIS group and 61 healthy subjects served as a control group in this study.TF mRNA and plasma TF antigen levels in monocytes were measured by RT-PCR and ELISA,respectively.Results The TF mRNA and plasma TF levels were significantly higher in AIS group at the acute phase and significantly lower in AIS group at the recovery stage than in control group(P<0.05).However,no significant difference was observed in plasma TF mRNA level between the two groups(P>0.05).Conclusion The blood is in a hypercoagulability and the risk of thrombosis increases in AIS patients at its attack stage.Dynamic observation of the TF mRNA and plasma TF levels in monocytes contributes to the understanding of its occurrence and development.