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| The Role of IκBα in TNF-α-induced Apoptosis in Hepatic Stellate Cell Line HSC-T6 | |
| 引用本文： | 瞿志军,罗端德,潘延风.The Role of IκBα in TNF-α-induced Apoptosis in Hepatic Stellate Cell Line HSC-T6[J].华中科技大学学报(医学英德文版),2007,27(4):407-410. |
| 作者姓名： | 瞿志军 罗端德 潘延风 |
| 作者单位： | Department of Infectious Diseases Longgang Central District Hospital，Department of Infectious Diseases，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department Infectious Diseases，the First Affiliated Hospital of Henan Medical University，Shenzhen 518116，China，Wuhan 430022，China，Zhengzhou 450022，China |
| 摘 要： | To investigate the role of NF-κB in TNF-α induced apoptosis in HSC-T6, a mutant IκBα was transfected into HSC-T6 cells by lipofectin transfection technique and its transient effect was examined 48 h after the transfection. The activation of NF-κB was detected by immune fluorescence cytochemistry and Western blotting with anti-p65 antibody. The apoptosis and the rate of inhibition by TNF-α in both transfected and untransfected HSC-T6 cells were measured respectively by FAC-Scan side scatter analysis and MTF methods. Our results showed that TNF-α could activate NF-κB in untransfected cells but not in transfected HSC-T6 cells. The percentage of apoptosis in transfected cells were significantly higher than that in the untransfected ones （P〈0.01） and it was also true of the inhibition rate （P〈0.01）. It is concluded that the resistance of HSC-T6 towards apoptosis induced by TNF-α can be mediated by NF-κB activation. The inhibition of NF-κB activation by mutant IκBα can attenuate the resistance of HSC-T6 cells and increase its sensitivity to TNF-α. |
| 关 键 词： | 核因子-κB 抑制蛋白 肿瘤坏死因子-α 肝疾病 |
| 收稿时间： | 9 January 2007 |
| 修稿时间： | 2007-01-09 |
The role of IκBα in TNF-α-induced apoptosis in hepatic stellate cell line HSC-T6 |
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| Qu?Zhijun,Lou?Duande,Pan?Yanfeng.The role of IκBα in TNF-α-induced apoptosis in hepatic stellate cell line HSC-T6[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(4):407-410. | |
| Authors: | Qu Zhijun Lou Duande Pan Yanfeng |
| Institution: | 1. Department of Infectious Diseases, Longgang Central District Hospital, Shenzhen 518116, China 2. Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 3. Department Infectious Diseases, the FirstAffiliated Hospital of Henan Medical University, Zhengzhou 450022, China |
| Abstract: | Summary To investigate the role of NF-κB in TNF-α induced apoptosis in HSC-T6, a mutant IκBα was transfected into HSC-T6 cells by lipofectin transfection technique and its transient effect was examined 48 h after the transfection. The activation of NF-κB was detected by immune fluorescence cytochemistry and Western blotting with anti-p65 antibody. The apoptosis and the rate of inhibition by TNF-α in both transfected and untransfected HSC-T6 cells were measured respectively by FAC-Scan side scatter analysis and MTT methods. Our results showed that TNF-α could activate NF-κB in untransfected cells but not in transfected HSC-T6 cells. The percentage of apoptosis in transfected cells were significantly higher than that in the untransfected ones (P<0.01) and it was also true of the inhibition rate (P<0.01). It is concluded that the resistance of HSC-T6 towards apoptosis induced by TNF-α can be mediated by NF-κB activation. The inhibition of NF-κB activation by mutant IκBα can attenuate the resistance of HSC-T6 cells and increase its sensitivity to TNF-α. QU Zhijun, male, born in 1971, Doctor in Charge |
| Keywords: | nuclear factor-κ B nuclear factor inhibiting protein tumor necrosis factor-α hepatic stellate cells apoptosis |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ | |