| Expression of Leptin Long-form Receptor mRNA in Luteinized Granulosa Cells of Obese Women with Polycystic Ovary Syndrome |
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| 作者姓名： | 尹婕 刘义 吕立群 王冬花 龚成 肖维 盛慧 |
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| 作者单位： | Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China |
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| 摘 要： | To investigate the expression of mRNA of leptin long-form receptor(OB-Rb) in lu-teinized granulosa cells of obese women with polycystic ovary syndrome(PCOS),and to determine the role of leptin in the physiopathology of PCOS,luteinized granulosa cells were collected from the follicle fluid of 10 obese women who met the diagnostic criteria for PCOS and their BMI was equal to or greater than 25 kg/m2,and at the same time,granulosa cells were collected from 10 normal women undergoing IVF-ET who served as the control group.Some luteinized granulosa cells were taken from normal women for in-vitro culture,into which human leptin of different concentrations was added(0,10,100 and 1000 ng/mL).After stimulation with leptin for 48 h,RT-PCR was em-ployed for the detection of the expression of OB-RLmRNA in the luteinized granulosa cells.Our re-sults showed that the level of OB-RLmRNA in luteinized granulosa cells of obese PCOS women was higher than those in the control(P<0.05).In luteinized granulosa cells cultured in vitro and stimulated by human leptin for 48 h,the level of OB-RLmRNA was higher than those without leptin stimulation(P<0.01),and when leptin concentration was at 100 ng/mL,and the level of OB-RLmRNA reached a peak.It is concluded that in obese PCOS women,the level of serum leptin is increased,which pro-motes the expression of OB-RL in luteinized granulosa cells and increases the sensitivity of the granulosa cells to leptin.Leptin may contribute to anovulation in obese women with PCOS
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| 关 键 词： | 多囊卵巢综合症 肿瘤细胞 肥胖 治疗 |
| 收稿时间： | 7 September 2006 |
| 修稿时间： | 2006-09-07 |
Expression of leptin long-form receptor mRNA in luteinized granulosa cells of obese women with polycystic ovary syndrome |
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| Yin?Jie,Liu?Yi,Lv?Liqun,Wang?Donghua,Gong?Cheng,Xiao?Wei,Sheng?Hui.Expression of Leptin Long-form Receptor mRNA in Luteinized Granulosa Cells of Obese Women with Polycystic Ovary Syndrome[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(4):461-463. |
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| Authors: | Yin Jie Liu Yi Lv Liqun Wang Donghua Gong Cheng Xiao Wei Sheng Hui |
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| Institution: | Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China |
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| Abstract: | Summary To investigate the expression of mRNA of leptin long-form receptor (OB-Rb) in luteinized granulosa cells of obese women with
polycystic ovary syndrome (PCOS), and to determine the role of leptin in the physiopathology of PCOS, luteinized granulosa
cells were collected from the follicle fluid of 10 obese women who met the diagnostic criteria for PCOS and their BMI was
equal to or greater than 25 kg/m2, and at the same time, granulosa cells were collected from 10 normal women undergoing IVF-ET who served as the control group.
Some luteinized granulosa cells were taken from normal women for in-vitro culture, into which human leptin of different concentrations was added (0, 10, 100 and 1000 ng/mL). After stimulation with
leptin for 48 h, RT-PCR was employed for the detection of the expression of OB-RLmRNA in the luteinized granulosa cells. Our
results showed that the level of OB-RLmRNA in luteinized granulosa cells of obese PCOS women was higher than those in the
control (P<0.05). In luteinized granulosa cells cultured in vitro and stimulated by human leptin for 48 h, the level of OB-RLmRNA was higher than those without leptin stimulation (P<0.01), and when leptin concentration was at 100 ng/mL, and the level of OB-RLmRNA reached a peak. It is concluded that in obese PCOS women, the level of serum leptin is increased, which promotes the
expression of OB-RL in luteinized granulosa cells and increases the sensitivity of the granulosa cells to leptin. Leptin may contribute to anovulation
in obese women with PCOS.
YIN Jie, female, born in 1981, Master of Medical Sciences |
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| Keywords: | polycystic ovary syndrome leptin leptin receptor granulosa cell |
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