突变型P~(53)基因在人乳腺癌组织中表达的研究

目的探讨突变型P53基因在人乳腺癌组织中的表达规律及其与淋巴结转移等之间的关系。方注乳腺组织标本76例，其中乳腺癌56例，乳腺纤维囊性病10例、乳腺纤维腺癌10例，采用SP免疫组织化学方法检测突变型PS’基因在乳腺组织中蛋白水平的表达。结果①突变型P53蛋白存在于胞核与胞浆，以胞核为主。②P53蛋白在乳腺癌组织中的表达率为35．71％，而在乳腺良性疾患中均为阴性。③P53蛋白的表达与乳腺癌淋巴结转移正相关，且在13对原发灶与淋巴结转移灶配对标本研究中有6对表达一致，其表达与组织病理类型及分化程度无关。结论P53基因突变在乳腺癌的发生及淋巴转赶上可能起重要作用，突变型P53蛋白的检测可用于估测乳腺癌患者淋巴结转移倾向。

A Study on expression of the mutated p53 gene in human breast cancer

To research expression of the mutated p53 gene in human breast cancers and its correlation with lymph node metastasis and other prognostic factors.Methods By using SP immunohistochemical methods, production of mutated p53 gene expression of 76 breast tissue samples was detected.Results ① Mutated P53 protein were in the nucleus and the cytoplasm, but in the nucleus chiefly. ② The frequency of p53 gene expression was 35. 71 % in the breast cancer,but negative in all breast benign lessions. ③ p53 protein expression was significantiy correlation with lymph nodalmetastasis of breast cancer. and no significant difference were found between primary and metastatic .ites in 6 of 13 paired samples of p53 expression,and p53 expression were not correlated with tumor cell differentiation, nor were it correlated with histologic tumor type.Conclusion p53 mutation may play an important role in neoplasia and lymph node metastasis of breast cancer and detection of mutated p53 protein may have certain importance in the evaluation of lympiiatic metastasis of breast cancers.