Carnitine content in the follicular fluid and expression of the enzymes involved in beta oxidation in oocytes and cumulus cells |
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| Authors: | Debbie Montjean Frida Entezami Isabelle Lichtblau Stephanie Belloc Timur Gurgan Yves Menezo |
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| Affiliation: | 1.UNILABS, Laboratire d’Eylau, 55 Rue St Didier, 75116 Paris, France ;2.Laboratoire Dynabio, Clinique du Cotentin, 50120 Equeurdreville, France ;3.The Gurgan clinic, Ankara, Turkey ;4.London fertility associates, Harley Street, London, UK |
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| Abstract: | Purpose The purpose of this study is to study lipid metabolism in oocytes and embryos that is a neglected parameter in human IVF. Methods We have tested the total carnitine content (TC) in the follicular fluid of 278 patients (217 non pregnant, 61 pregnant) undergoing IVF. Results The follicular fluid TC is neither correlated with the circulating estradiol content in serum nor with the outcome the IVF attempt. Carnitine, through the carnitine shuttle, is a major partner in lipid beta oxidation, metabolic pathway involved in the acquisition of oocyte competence. The expression of carnitine synthesis enzymes and lipid beta oxidation was studied in cumulus cells collected at the time of ovum retrieval and in oocyte. Surprisingly the expression for carnitine synthesis is not detectable in oocytes whereas the enzymes involved in lipid beta oxidation are rather strongly expressed. Conclusions The addition of carnitine in oocyte maturation and embryo culture media should not be overlooked. |
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| Keywords: | Human follicular fluid Oocyte Carnitine Lipid betaoxidation Mitochondria |
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