|
|||||
|
|
Oxidative damages in the DNA, lipids, and proteins of rats exposed to isofluranes and alcohols |
|
| Authors: | Kim Heezoo Oh Eunha Im Hosub Mun Joohee Yang Minho Khim Jin-Young Lee Eunil Lim Sang Ho Kong Myoung Hoon Lee Mikyoung Sul Donggeun |
| Affiliation: | aDepartment of Anesthesiology and Pain Medicine, Guro Medical Center, Korea University, Gurodong 80, Guro-gu, Seoul, South Korea bEnvironmental Toxico-Genomic and Proteomic Medical Center, College of Medicine, Korea University, Anamdong 5, Sungbukku, Seoul 136-701, South Korea cSchool of Public Health, Korea University, Anamdong 5, Sungbukku, Seoul 136-701, South Korea dDepartment of Preventive Medicine, College of Medicine and Institute for Environmental Health, Medical Science Research Center, Korea University, 5 anamdong Sungbukku, Seoul 136-701, South Korea eGraduate School of Medicine, Korea University, Anamdong 5 Ka, Sungbukku, Seoul 136-701, South Korea |
| Abstract: | DNA damage, lipid peroxidation and protein oxidation were evaluated in rats exposed to a 1% isoflurane atmosphere with or without alcohol administration (administrated by gastric intubation at 4 g/kg body weight as a 50% solution). Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the lymphocytes, spleen, bone marrow, brain, livers and lung of rats exposed to 1% isoflurane for 30 or 60 min with/without ethanol. Levels of malondialdehydes (MDA), a metabolite of lipid peroxidation, were determined in plasma and tissues. Carbonyl contents were also analyzed to determine levels of protein oxidation in plasma and tissues. Levels of DNA damage in lymphocytes, bone marrow, and the organ tissues of rats exposed to isoflurane were found to increase time dependently, and alcohol increased DNA damage. Lipid peroxidation and protein oxidation results showed patterns that differed from those of DNA damage. Levels of MDA in plasma, bone marrow, spleen, and the livers of rats exposed to isoflurane with/without ethanol were found to be time dependently increased, but this was not observed in the brain or lung. However, protein oxidation levels were significantly increased in the plasma, brains, and lungs of rats exposed to isoflurane, and exposure to isoflurane and alcohol, significantly increased these levels in plasma and brain. The present study demonstrates that isoflurane exposure results in significant DNA damage in rat lymphocytes, bone marrow, spleen, brain, livers, and lung. Moreover, alcohol was found to be as a strong inducer of DNA damage, lipid peroxidation and protein oxidation. However, no evidence in association between DNA damage, lipid peroxidation and protein oxidation was found. Regarding the effects of isoflurane and alcohol on oxidative damages, single strand DNA damages may be a useful biomarkers and blood cells and plasma appear to be more sensitive targets to oxidative damage than other tissues. |
| Keywords: | Alcohol Bone marrow Brain Comet assay DNA damage Isoflurane Lipid peroxidation Liver Lung Lymphocytes Malondialdehyde Plasma Protein oxidation Single strand DNA breakage Spleen |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |