丹龙定喘汤对哮喘豚鼠气道重塑的作用及机制研究

目的初步探析丹龙定喘汤对哮喘豚鼠气道重塑的作用及机制。方法选取健康雌性豚鼠24只,按体质量分层随机分为4组：空白对照组、模型对照组、地塞米松组、丹龙定喘汤组,每组6只。采用卵蛋白致敏法建立豚鼠哮喘气道重塑模型,以丹龙定喘汤干预,并以地塞米松做对照药物,治疗1个月后分别检测气道重塑的程度及豚鼠肺组织中转化生长因子β1（TGF-β1）含量。结果 （1）模型对照组豚鼠肺支气管管壁和平滑肌层明显增厚,与空白对照组相比差异有统计学意义（P〈0.05）;丹龙定喘汤组气道壁厚度减轻,与模型对照组相比差异有统计学意义（P〈0.05）,与地塞米松组相比,差异无统计学意义（P〉0.05）。（2）模型对照组豚鼠肺组织中的TGF-β1含量明显增加,与空白对照组相比,差异有统计学意义（P〈0.05）;丹龙定喘汤组TGF-β1含量减少,与模型对照组相比差异有统计学意义（P〈0.05）,与地塞米松组相比差异无统计学意义（P〉0.05）。结论丹龙定喘汤对哮喘豚鼠气道重塑有一定抑制作用,其机制可能是通过下调哮喘豚鼠肺组织中的TGF-β1水平,抑制气道平滑肌细胞及细胞外基质的增殖而阻断气道重塑的进程。

The research of effectiveness and mechanism of Danlong panting-arresting decoction in airway remodeling in asthmatic guinea pig

Objective To make a preliminary exploration of the effectiveness and mechanism of Danlong panting-arresting decoction in airway remodeling in asthmatic guinea pig.Methods Twenty-four healthy female guinea pigs were divided into four groups randomly（n=6,in each group）：normal control group,model control group,dexamethasone group,and Danlong panting-arresting decoction group.The asthmatic guinea pig airway remodeling model was established by ovalbumin sensitized method,Danlong panting-arresting decoction was applied to intervene,and dexamethasone was used as control drug.After one month,detect the extent of airway remodeling and determine TGF-β1 level in guinea pig lung tissues.Results（1）Compared with normal group,WAt/Pi,WAm/Pi were significantly increased（P0.05）in model group.Compared with model group,the airway wall thickness reduced in Danlong panting-arresting decoction group,there being a significant difference（P0.05）,but no significant difference compared with dexamethasone group（P0.05）.（2）Compared with normal group,the extent of lung tissue TGF-β1 was significantly increased in the model group（P0.05）.Compared with model group,the TGF-β1 content was decreased in Danlong panting-arresting decoction group,there being a significant difference（P0.05）,but no significant difference compared with the dexamethasone group（P0.05）.Conclusions Danlong panting-arresting decoction plays an inhibitory role in airway remodeling of asthmatic guinea pigs.It may reduce the TGF-β1 level of lung tissues in asthmatic guinea pigs,thus inhibit airway proliferation of smooth muscle cells and extracellular matrix and block the airway remodeling process.