兔骨髓基质细胞的分离培养

目的 为骨组织工程寻找一种理想的种子细胞。方法 采取兔骨髓组织，应用梯度离心获取骨髓基质细胞，体外培养传代，通过光镜、透射电镜及成骨特性的鉴定，观察细胞的形态、生长特点及成骨特性。结果 培养的骨髓基质细胞形态多为三角形或梭形，生长增殖迅速，具有成骨能力，易于定向分化为成骨细胞。结论 自骨髓获得的骨髓基质细胞具有明显的增殖能力和成骨活性，可以做为骨组织工程中比较理想的种子细胞。

Separating cultivating and osteogeneric identifying of bone marrow stromal cells in rabbit

Objective To search a kind of ideal seeded cell of bone tissue engineering. Methods Bone marrow tissue was fetched from rabbit,got bone marrow stromal cells by the method of density centrifuge,observed the conformation,growing feature and osteogeneric capability of the cells during culture in vitro by light microscope,transmission electron microscope and measure of osteogeneric capability. Results Most of the cultivated bone marrow stromal cells were triangle-like or shuttle-like cells. They growed and breeded rapidly,and had osteogeneric capability. They were easy to differentiate to osteoblasts. Conclusion The cultivated bone marrow stromal cells are matured ones,and could become the more ideal seeded cells for bone tissue engineering.