基因芯片检测与苯中毒相关的信号传导通路中基因的差异表达

目的检测苯中毒信号转导相关基因的表达，探讨苯引起的血液系统损害的发病机理。方法应用含4265条信号转导基因的cDNA芯片检测7例苯中毒患者和7例无苯接触的正常人的外周血白细胞基因表达谱，观察其在基因表达谱上的差异。进行了基因表达谱聚类分析。结果在4265条基因中，存在明显差异表达的基因176条。至少在六张芯片结果中有显著性表达上调的基因35个，主要包括有PTPRC、STAT4、IFITM1等。至少在5张芯片结果中有显著性表达下调的基因45个，主要包括有ARHB、PPP3CB、CDC37等。结论微矩阵基因芯片在检测苯中毒信号转导相关基因的改变时．具有快速、高通量、高灵敏度等特点．细胞信号转导的障碍在苯中毒发病中起一定的作用。

Differentially expressed genes of cell signal transduction associated with benzene poisoning by cDNA microarray

Objective To detect the differential expression of cell signal transduction genes associated with benzene poisoning,and to explore the pathogenic mechanisms of blood system damage induced by benzene .Methods Peripheral white blood cell gene expression profile of 7 benzene poisoning patients, including one aplastic anemia,was determined by eDNA microarray. Seven chips from normal workers were served as controls. Cluster analysis of gene expression profile was performed. Results Among the 4265 target genes, 176 genes associated with cell signal transduction were differentially expressed . 35 up-regulated genes including PTPRC, STAT4, IFITM1 etc were found in at least 6 pieces of microarray ;45 down-regulated genes including ARHB, PPP3CB, CDC37 etc were found in at least 5 pieces of microarray. Conclusion eDNA microarray technology is an effective technique for screening the differentially expressed genes of cell signal transduction. Disorder in cell signal transduction may play certain role in the pathogenic mechanism of benzene poisoning.