Fixation of pharmacologically active amines on polyphosphonates, 3. Synthesis and preliminary in vitro cytotoxic studies of bis(2-chloroethyl)amino group containing polyphosphonates

Hydrosoluble poly(3,6,9-trioxaundecamethylene phosphonate) with attached cytotoxic bis(2-chloroethyl)amine in the side chain was synthesized by chemical modification of the corresponding polyphosphonate via the Atherton-Todd reaction. The structure of the product was confirmed by ¹H, ¹³C and ³¹P NMR spectroscopy. Preliminary in vitro cytotoxic studies revealed that the polymeric derivative exhibited an eight fold more cytotoxic potency against human hepatocellular carcinoma cell line HepG2 than against murine leukemia cell line L1210.