Labeling of hematoporphyrin with 105Rh and binding studies with human gamma globulin期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Labeling of hematoporphyrin with 105Rh and binding studies with human gamma globulin

Affiliation:	1. Center for Radiopharmaceutical Sciences ETH-PSI-USZ, Paul Scherrer Institute, Villigen-PSI, Switzerland;2. Laboratory of Radiochemistry and Environmental Chemistry, Paul Scherrer Institute, Villigen-PSI, Switzerland;3. Laboratory of Radiochemistry and Environmental Chemistry, Department of Chemistry and Biochemistry University of Bern, Bern, Switzerland;4. Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland;1. Department of Molecular Oncology, BC Cancer Agency, Vancouver, BC, Canada;2. Life Sciences Division, TRIUMF, Vancouver, BC, Canada;3. Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, BC, Canada;4. Department of Radiology, University of British Columbia, Vancouver, BC, Canada;5. Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada;6. Department of Functional Imaging, BC Cancer Agency, Vancouver, BC, Canada

Abstract:	Labeling of hematoporphyrin with ¹⁰⁵Rh at stoichiometric concentrations is described. Labeling efficiencies of up to 93% could be obtained at pH 9.0 in bicarbonate buffer. Solvent extraction of ¹⁰⁵Rhhematoporphyrin into methyl isobutyl ketone was used to estimate the complex yield. The complex showed high stability and no loss of ¹⁰⁵Rh was seen throughout the 6 days of study. ¹⁰⁵Rhhematoporphyrin when incubated with human γ globulin was seen to be quantitatively bound to the protein. This procedure may be used for labeling monoclonal antibodies with ¹⁰⁵Rh for therapeutic applications.

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