Selection of anti-F protein B-cell repertoires in normal mice |
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Institution: | 1. School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, China;2. Department of Materials Science and Engineering, Hong Kong Institute for Advanced Study, City University of Hong Kong, Kowloon, Hong Kong SAR, China;3. Department of Materials and Optoelectronic Science, National Sun Yat-Sen University, Kaohsiung, Taiwan |
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Abstract: | The hypothesis that self-tolerance to F protein antigen exclusively concerns T cells was tested by determining the frequencies of B lymphocytes producing anti-F antibodies in bone marrow (BM), spleen and peritoneal exudate (PEC) cells from normal, immune or tolerant animals, and in responder and non-responder mouse strains. Using an ELISA spot assay and lipopolysaccharide stimulation, we found that anti-F frequencies were highest in BM and “naturally activated” large spleen cells, followed by resting spleen and PEC cells. Anti-F specificities were also induced among “natural” Ig-secreting cells of normal individuals. Specific immunization of responder mice doubled the splenic frequencies, while tolerization had no effect. Similar results were obtained in BALB/c and A/J mice, while C57BL/6 contained fewer anti-F B cells in spleen, but not in BM. These results support the notion that self-tolerance to F antigen can primarily be ascribed to T cells, but they also show F-antigen-specific selection of B-cell repertoires. |
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