MicroRNA-374a promotes pancreatic cancer cell proliferation and epithelial to mesenchymal transition by targeting SRCIN1 |
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| Affiliation: | 1. Department of General Surgery, Tianjin Fifth Central Hospital, Tianjin, 300450, China;2. Department of General Surgery, the First Affiliated Hospital of Jiamusi University, Jiamusi, 154003, China;1. Department of Pathology, Kyung Hee University Hospital at Gangdong, School of Medicine, Kyung Hee University, Seoul, South Korea;2. Department of Surgery, Kyung Hee University Hospital at Gangdong, School of Medicine, Kyung Hee University, Seoul, South Korea;3. Department of Pathology, School of Medicine, Chungnam National University, Daejeon, South Korea;1. Department of Pharmacology, Physiology and Neuroscience, University of South Carolina, Columbia, SC, United States;2. Department of Pharmaceutical and Administrative Sciences, Presbyterian College School of Pharmacy, Clinton, SC, United States;1. Department of Endocrinology and Metabolism, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China;2. Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China;1. Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA;2. Department of Obstetrics and Gynecology, and Reproductive Science, University of Pittsburgh, PA 15213, USA;3. Department of Pediatrics, University of Pittsburgh, Pittsburgh, PA 15219, USA;4. Division of Animal Sciences and Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA;5. Department of Biochemistry and Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA;6. Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15219, USA;1. Yangzhou University Medical College, Yangzhou University, Yangzhou, Jiangsu Province 225009, China;2. Department of Obstetrics, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu Province 225012, China;3. Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, Yangzhou, Jiangsu Province 225009, China |
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| Abstract: | MicroRNAs (miRNAs) play a vital role in the progress of cancer. Whereas the expression and function of miR-374a in pancreatic cancer remain largely unknown. In this study, pancreatic cancer samples and its adjacent normal tissues were obtained from 30 clinical patients with pancreatic cancer. Quantitative real-time PCR (qRT-PCR) was used to measure miR-374a and SRC Kinase Signaling Inhibitor 1 (SRCIN1) expression. Western blotting assay was performed to measure the levels of SRCIN1, E-cadherin, N-cadherin, Vimentin, Zonula occludens-1 (ZO-1) and β-catenin in PANC-1 cells. Luciferase reporter assay was conducted to confirm the direct targeting of SRCIN1 by miR-374a. Cell proliferation and migration assays were utilized to analyze the role of miR-374a in PANC-1 cells. We found that miR-374a expression was upregulated in pancreatic cancer tissues and cell lines. Over-expression of miR-374a promoted cell proliferation, migration and epithelial-mesenchymal transition (EMT) in pancreatic cancer. While, SRCIN1 expression was downregulated in pancreatic cancer tissues and cells. SRCIN1 was found to be a potential targets of miR-374a by dual-luciferase reporter assay. And SRCIN1 was down-regulated after miR-374a transfection. More than that, over-expression of SRCIN1 inhibited cell proliferation, migration and EMT in pancreatic cancer cell. Therefore, this study revealed that miR-374a promoted cell proliferation, migration and EMT via targeting SRCIN1 in pancreatic cancer. |
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| Keywords: | microRNA miR-374a SRCIN1 Pancreatic cancer Epithelial-mesenchymal transition |
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