B subset cells in patients with chronic granulomatous disease in a Mexican population |
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| Affiliation: | 1. Department of Allergy and Clinical Immunology, Pediatric Hospital of Mexico “Federico Gómez”, Mexico City, Mexico;2. Immunodeficiencies Research Unit, National Institute of Pediatrics, Mexico City, Mexico;3. Clinical Immunology Department, National Institute of Pediatrics, Mexico City, Mexico;4. Department of Research and Methodology, National Institute of Pediatrics, Mexico City, Mexico;5. Allergy and Clinical Immunology Department, Pediatric Hospital of Chihuahua, Chihuahua, Mexico;6. Western National Medical Center, Department of Allergy and Clinical Immunology, Guadalajara, Jalisco, Mexico;1. Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children''s Medical Center, Tehran University of Medical Science, Tehran, Iran;2. Research Center for Primary Immunodeficiencies, Iran University of Medical Science, Tehran, Iran;3. Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, Stockholm, Sweden;1. Department of Pharmacology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan;2. Department of Urology, Kitasato University School of Medicine, Kanagawa 252-0374, Japan;3. Department of Microbiology, Kitasato University School of Allied Health Science, Kanagawa 252-0373, Japan;4. Laboratory of Host Defense, Osaka University, Osaka 565-0871, Japan;1. APHP Hôpital Trousseau;2. Sorbonne Université;1. Division of Allergy, Pulmonary, and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee;2. Division of Infectious Diseases, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee;3. Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, Western Australia;4. Division of Allergy, Immunology, and Pulmonary Medicine, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee;1. Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;2. Molecular and Cell Biology Center, Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;3. Pediatric Infectious Diseases Research Center, Mazandaran University of Medical Sciences, Sari, Iran |
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| Abstract: | IntroductionChronic granulomatous disease (CGD) is a disorder of phagocyte function, characterized by pyogenic infections and granuloma formation caused by defects in NADPH oxidase complex activity. Although the effect of CGD mainly reflects the phagocytic compartment, B cell responses are also impaired in patients with CGD.Materials and methodsFlow cytometric analysis was performed on peripheral blood samples from 35 CGD patients age-matched with healthy controls (HC). The target cells of our study were the naive (IgD+/CD27−), memory (IgD−/CD27+), and B1a (CD5+) cells. Immunoglobulins (Igs) were also measured. This study was performed in a Latin American cohort.ResultsWe found significantly higher levels of naive B cells and B1a cells, but lower levels of memory B cells were found in CGD patients compared to HC. There was no significant difference of cell percentages per inheritance type.DiscussionOur findings suggest that the deficiency of NADPH oxidase components can affect the differentiation of naive B cells to memory B cells. Consequently, memory cells will be low, which also influenced the expression of CD27 in memory B cells and as a result, the percentage of naive cells increases. An altered phenotype of B lymphocytes in CGD patients may contribute to the opportunistic infections and autoimmune disorders that are seen in this disease. |
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| Keywords: | Chronic granulomatous disease B cell subsets Long-term memory Memory B-cell compartment Reactive oxygen species deficiency B1 lymphocyte |
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