| 伤寒杆菌抗原酶免法、LPS-PHA在伤寒早期诊断中的应用 |
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| 引用本文： | 王伟,马华,李杰,李曼. 伤寒杆菌抗原酶免法、LPS-PHA在伤寒早期诊断中的应用[J]. 实用全科医学, 2005, 3(4): 367-367 |
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| 作者姓名： | 王伟 马华 李杰 李曼 |
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| 作者单位： | 安徽省蚌埠医学院医学检验系免疫学教研室，安徽省蚌埠医学院医学检验系免疫学教研室，蚌埠医学院附属医院检验科，山东省临沂市中医医院检验科 233003，233003 |
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| 摘 要： | 目的探讨伤寒杆菌抗原酶免法(ELISA)、脂多糖-被动血凝试验(LPS-PHA)在伤寒早期诊断中的应用价值。方法选择224例门诊发热疑似伤寒患者,于发病第3、4、7d,分别用ELISA法、LPS-PHA、血培养、肥达氏反应检测伤寒杆菌。结果伤寒杆菌抗原酶免法(ELISA)、LPS-PHA在第7d的检出率分别为85.4%和80.5%,显著高于血培养和肥达氏反应(P<0.05)。结论伤寒杆菌抗原酶免法(ELISA)、LPS-PHA在伤寒早期诊断中具有较高的应用价值,但二者都有一定的假阳性和假阴性,联合应用对伤寒的早期辅助诊断更有价值。
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| 关 键 词： | LPS-PHA 伤寒杆菌抗原 早期诊断 酶免法 肥达氏反应 被动血凝试验 ELISA法 早期辅助诊断 应用价值 方法选择 脂多糖 血培养 检出率 假阴性 假阳性 |
| 修稿时间： | 2004-12-01 |
Apply of Immunoassay of Typhoid Bacillus Antigen and LPS-PHA on the Early Diagnosis of Typhoid |
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| WANG Wei,MA Hua,LI Jie,et al.. Apply of Immunoassay of Typhoid Bacillus Antigen and LPS-PHA on the Early Diagnosis of Typhoid[J]. Applied Journal Of General Practice, 2005, 3(4): 367-367 |
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| Authors: | WANG Wei MA Hua LI Jie et al. |
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| Affiliation: | WANG Wei,MA Hua,LI Jie,et al. Department of Immunology,Bengbu Medical College,Anhui,233003 |
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| Abstract: | Objective To explore the value of immunoassay of typhoid antigen(ELISA) and lipopolysaccharide-passive hemagglutination assay (LPS-PHA) on the early diagnosis of typhoid. Methods 224 suspicious outpatients with typhoid were tested by ELISA, LPS-PHA, blood culture, Widal test on 3rd day, 4th day, 7th day. Results The positive rate of immunoassay of typhoid (ELISA) and LPS-PHA in typhoid individuals were 85.4%, 80.5% on 7th day, both of them were much higher than those of blood culture and Widal test (P<0.05). Conclusions ELISA and LPS-PHA have the good value on early diagnosis of typhoid, but both of them have false positive and false negative. Associated application of ELISA and LPS-PHA for early diagnosis of typhoid has a higher applied value. |
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| Keywords: | Typhoid LPS-PHA ELISA |
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